GST amplification by PCR
(150-250 bp of the 3'UTR)
Biological Samples :
- 3 independent hybridizations using 3 different RNA pools
- Each RNA pool from 3 independent plants
Spotting on nylon membrane
Biorobotics
MicrogridII
Total RNA extraction
P33 cDNA probe
Hybridization
Exposure
Image analysis (Imagequant software)
Data analysis (normalization, statistical analysis)
Informative representation of data (clusterization, HCE2 software)
In-depth data analysis

Results

      (gene expression/gene/condition)