TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCN21b10.yg.2.1
(574 letters)
Database: Sorghum_nucl_with_EST.fasta
832,831 sequences; 491,359,669 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF466200.2| Sorghum bicolor putative protein kinase gene... 105 1e-076
gb|CN127712.1|CN127712 RHOH1_24_D05.g3_A002 Acid- and alkal... 264 8e-070
gb|CF484299.1|CF484299 POL1_4_A06.g1_A002 Pollen Sorghum bi... 261 7e-069
gb|CF488926.1|CF488926 POL1_53_C07.b1_A002 Pollen Sorghum b... 191 7e-065
gb|CW486925.1|CW486925 fsbb001f252h17f0 Sorghum methylation... 85 4e-060
gb|CW173784.1|CW173784 104_585_11157034_148_36551_037 Sorgh... 80 9e-046
gb|CW098046.1|CW098046 104_464_11002794_148_34367_073 Sorgh... 85 3e-035
gb|CW042123.1|CW042123 104_276_10506948_114_30476 Sorghum m... 84 4e-034
gb|CW437404.1|CW437404 fsbb001f154i03k0 Sorghum methylation... 84 4e-034
gb|CW111980.1|CW111980 104_485_11104632_116_34538_086 Sorgh... 84 4e-034
gb|CW209770.1|CW209770 104_640_11189431_116_37430_032 Sorgh... 86 1e-033
gb|BG159208.1|BG159208 RHIZ2_27_F09.b1_A003 Rhizome2 (RHIZ2... 129 3e-029
gb|CF489000.1|CF489000 POL1_53_C07.g1_A002 Pollen Sorghum b... 92 6e-029
gb|CW486926.1|CW486926 fsbb001f252h17k0 Sorghum methylation... 85 2e-028
gb|BI098036.1|BI098036 IP1_31_C06.g1_A002 Immature pannicle... 121 1e-026
gb|CW044337.1|CW044337 104_280_10508891_115_30242 Sorghum m... 105 8e-026
gb|CF484228.1|CF484228 POL1_4_A06.b1_A002 Pollen Sorghum bi... 111 7e-024
gb|BF656435.1|BF656435 FM1_45_G12.g2_A003 Floral-Induced Me... 109 3e-023
gb|CW241144.1|CW241144 104_700_11218605_148_37542_093 Sorgh... 87 1e-022
gb|CL176804.1|CL176804 104_383_10893374_116_31773_206 Sorgh... 80 4e-022
gb|CW098045.1|CW098045 104_464_11002794_116_34363_073 Sorgh... 105 6e-022
>gb|AF466200.2| Sorghum bicolor putative protein kinase gene, partial cds; putative Cf-2,
fertilization-independent endosperm proteins, hypothetical
protein, putative non-LTR retroelement reverse
transcriptase, OCL5 protein, tryptophan synthase
beta-subunit, hypothetical proteins, putative AP
endonuclease, putative RNA polymerase II complex component
SRB7, putative beta-1,3-glucanase, hypothetical protein,
TNP2-like protein, hypothetical protein, putative
phosphate/phosphoenolpyruvate translocator, putative
protein, hypothetical proteins, putative
galactosyltransferase family, hypothetical protein,
putative cytochrome P450 family, putative lipid transfer
protein, putative photoreceptor-interacting protein, and
hypothetical protein genes, complete cds; and hypothetical
protein gene, partial cds
Length = 202197
Score = 105 bits (224), Expect(5) = 1e-065
Identities = 41/47 (87%), Positives = 42/47 (89%)
Frame = +2 / +2
Query: 47 EFEEGGQCNVEERSRILWRHCHFAIYRPL*AGCS*DNCYL*VWGPEL 187
EF+E GQCN EERSRILWRHCHFAIYR L*AGCS*DNCYL*VWG L
Sbjct: 170150 EFKERGQCNAEERSRILWRHCHFAIYRSL*AGCS*DNCYL*VWGKTL 170290
Score = 105 bits (224), Expect(5) = 1e-076
Identities = 44/46 (95%), Positives = 46/46 (100%)
Frame = +3 / +3
Query: 48 NSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGVQN 185
NSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGV++
Sbjct: 170151 NSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGVRH 170288
Score = 104 bits (221), Expect(5) = 7e-071
Identities = 39/41 (95%), Positives = 40/41 (97%)
Frame = -3 / -2
Query: 176 PILTDSNCLKNNQLIAVYKWQNDNVSKVFCFFLQHYIDLLP 54
PILTDSNCLKNNQLIA+YKWQNDNVSKVFCFFLQHYIDL P
Sbjct: 170279 PILTDSNCLKNNQLIAIYKWQNDNVSKVFCFFLQHYIDLFP 170157
Score = 98.7 bits (209), Expect(5) = 4e-062
Identities = 44/47 (93%), Positives = 45/47 (95%)
Frame = -1 / -3
Query: 187 KFWTPYSQIAIVLRTTSS*RSINGKMTMSPKYSASFFNITLTSFLEF 47
K TPYSQIAIVLRTTSS*RSINGKMTMSPKYSASFF+ITLTSFLEF
Sbjct: 170290 KCLTPYSQIAIVLRTTSS*RSINGKMTMSPKYSASFFSITLTSFLEF 170150
Score = 93.2 bits (197), Expect(5) = 3e-052
Identities = 40/43 (93%), Positives = 42/43 (97%)
Frame = +1 / +1
Query: 49 IRGRRSM*C*RKKQNTLETLSFCHL*TAMSWLFLRQLLSVSMG 177
I+G+RSM*C*RKKQNTLETLSFCHL* AMSWLFLRQLLSVSMG
Sbjct: 170152 IQGKRSM*C*RKKQNTLETLSFCHL*IAMSWLFLRQLLSVSMG 170280
Score = 84.9 bits (179), Expect(5) = 1e-076
Identities = 32/34 (94%), Positives = 33/34 (97%)
Frame = +3 / +2
Query: 348 EEWPEDIYPPYANGPGYVISGDIAKFIVSQRANQ 449
+EWPEDIYPPYANGPGYVISGDIAKFIVSQ ANQ
Sbjct: 170933 QEWPEDIYPPYANGPGYVISGDIAKFIVSQHANQ 171034
Score = 80.8 bits (170), Expect(5) = 7e-071
Identities = 31/34 (91%), Positives = 32/34 (94%)
Frame = -2 / -2
Query: 450 SDWHAVTR*ILLCHQKSHSLVRLHMAGRYPQATP 349
SDWHAVTR*ILLCH+KSHSLV LHM GRYPQATP
Sbjct: 171035 SDWHAVTR*ILLCHRKSHSLVHLHMVGRYPQATP 170934
Score = 78.5 bits (165), Expect(5) = 4e-062
Identities = 30/34 (88%), Positives = 31/34 (91%)
Frame = -3 / -3
Query: 449 LIGTL*HDEFCYVTRNHIAWSVCIWRVDILRPLL 348
LIG L*HDEFCYVT NHIAWS+CIW VDILRPLL
Sbjct: 171034 LIGML*HDEFCYVTGNHIAWSICIWWVDILRPLL 170933
Score = 74.8 bits (157), Expect(5) = 1e-065
Identities = 31/34 (91%), Positives = 31/34 (91%)
Frame = +2 / +1
Query: 350 GVA*GYLPAICKRTRLCDFW*HSKIHRVTACQSE 451
GVA*GYLP ICK TRLCDF *HSKIHRVTACQSE
Sbjct: 170935 GVA*GYLPTICKWTRLCDFR*HSKIHRVTACQSE 171036
Score = 73.9 bits (155), Expect(5) = 1e-076
Identities = 28/29 (96%), Positives = 28/29 (96%)
Frame = +3 / +2
Query: 264 NNGDKPLYMGNLNLLHRPLRTGKWAVTDE 350
NNGDKPLYMGNLNLLHRPLRTGKWAVT E
Sbjct: 170687 NNGDKPLYMGNLNLLHRPLRTGKWAVTGE 170773
Score = 70.7 bits (148), Expect(5) = 1e-076
Identities = 29/31 (93%), Positives = 30/31 (96%)
Frame = +3 / +2
Query: 177 VQNLTAANIMKCDDDTFVRVDVVLRRIKLNN 269
VQNLTAANIMKCDDDTFVRVD+VLR IKLNN
Sbjct: 170597 VQNLTAANIMKCDDDTFVRVDMVLRHIKLNN 170689
Score = 70.7 bits (148), Expect(5) = 7e-071
Identities = 27/31 (87%), Positives = 29/31 (93%)
Frame = -3 / -3
Query: 269 IVQLDASQNYIYSHKCIIVTFHDVCSSQVLD 177
I+QLD SQN+IYSHKCIIV FHDVCSSQVLD
Sbjct: 170689 IIQLDVSQNHIYSHKCIIVAFHDVCSSQVLD 170597
Score = 70.3 bits (147), Expect(5) = 1e-065
Identities = 27/30 (90%), Positives = 27/30 (90%)
Frame = +2 / +1
Query: 176 GPELDCCKHHEM*R*YICESRCSSETHQVE 265
GPELDCCKHHEM R*YICESR SETHQVE
Sbjct: 170596 GPELDCCKHHEMRR*YICESRYGSETHQVE 170685
Score = 69.3 bits (145), Expect(5) = 3e-052
Identities = 30/34 (88%), Positives = 31/34 (91%)
Frame = +1 / +3
Query: 349 RSGLRISTRHMQTDQAM*FLVT*QNSSCHSVPIR 450
RSGLRIST HMQ DQAM*F VT*QNSSCHS+PIR
Sbjct: 170934 RSGLRISTHHMQMDQAM*FPVT*QNSSCHSMPIR 171035
Score = 69.3 bits (145), Expect(5) = 7e-071
Identities = 25/29 (86%), Positives = 27/29 (93%)
Frame = -3 / -3
Query: 350 LVCNCPFSSPQWSVQEVKVPHI*WFVTIV 264
L CNCPFSS QWS+QEVKVPHI*WFVTI+
Sbjct: 170773 LACNCPFSSSQWSMQEVKVPHI*WFVTII 170687
Score = 68.9 bits (144), Expect(5) = 4e-062
Identities = 29/31 (93%), Positives = 29/31 (93%)
Frame = -1 / -1
Query: 268 LFNLMRLRTTSTLTNVSSSHFMMFAAVKFWT 176
LFNLM LRT STLTNVSSSHFMMFAAVKFWT
Sbjct: 170688 LFNLMCLRTISTLTNVSSSHFMMFAAVKFWT 170596
Score = 66.1 bits (138), Expect(4) = 1e-030
Identities = 28/33 (84%), Positives = 30/33 (90%)
Frame = -2 / -2
Query: 273 HHCST*CVSELHLLSQMYHRHIS*CLQQSSSGP 175
H+ ST*CVSE +LLSQMYHR IS*CLQQSSSGP
Sbjct: 170693 HYYST*CVSEPYLLSQMYHRRIS*CLQQSSSGP 170595
Score = 64.8 bits (135), Expect(5) = 1e-065
Identities = 25/28 (89%), Positives = 25/28 (89%)
Frame = +1 / +3
Query: 268 MVTNHYIWGTLTSCTDH*GLENGQLQTR 351
MVTNHYIWGTLTSC DH* LENGQLQ R
Sbjct: 170691 MVTNHYIWGTLTSCIDH*ELENGQLQAR 170774
Score = 61.6 bits (128), Expect(5) = 1e-076
Identities = 24/33 (72%), Positives = 25/33 (75%)
Frame = +3 / +2
Query: 459 LFXMEDVSMGLWVEKFNSTXXVXYSHSWKXXXY 557
LF MEDVSMGLWVEKFN+T V YSHSW Y
Sbjct: 171125 LFKMEDVSMGLWVEKFNATKPVQYSHSWNFCQY 171223
Score = 59.7 bits (124), Expect(5) = 3e-052
Identities = 24/28 (85%), Positives = 25/28 (89%)
Frame = +2 / +1
Query: 269 W*QTIIYGEP*PPAQTIEDWKMGSYRRG 352
W*QTIIYGEP*P A T E+WKMGSYRRG
Sbjct: 170692 W*QTIIYGEP*PLA*TTENWKMGSYRRG 170775
Score = 57.4 bits (119), Expect(5) = 4e-062
Identities = 24/28 (85%), Positives = 25/28 (89%)
Frame = -2 / -2
Query: 351 PRL*LPIFQSSMVCAGG*GSPYIMVCHH 268
PRL*LPIFQ S+V A G*GSPYIMVCHH
Sbjct: 170774 PRL*LPIFQFSVVYARG*GSPYIMVCHH 170691
Score = 57.4 bits (119), Expect(4) = 1e-030
Identities = 24/25 (96%), Positives = 24/25 (96%)
Frame = -1 / -1
Query: 451 L*LARCDTMNFAMSPEIT*PGPFAY 377
L*LA CDTMNFAMSPEIT*PGPFAY
Sbjct: 171036 L*LACCDTMNFAMSPEIT*PGPFAY 170962
Score = 55.1 bits (114), Expect(4) = 1e-030
Identities = 24/25 (96%), Positives = 24/25 (96%)
Frame = -2 / -1
Query: 177 PHTHR*QLS*EQPAHSGL*MAK*QC 103
PHTHR*QLS*EQPAHS L*MAK*QC
Sbjct: 170280 PHTHR*QLS*EQPAHSDL*MAK*QC 170206
Score = 52.4 bits (108), Expect(5) = 7e-071
Identities = 23/33 (69%), Positives = 24/33 (72%)
Frame = -1 / -1
Query: 556 YXXXFQLXEYXTXXVELNFSTHRPMLTSSIXNS 458
Y FQL EY T V LNFSTHRP+LTSSI NS
Sbjct: 171222 YWQKFQLWEYWTGFVALNFSTHRPILTSSILNS 171124
Score = 48.7 bits (100), Expect(5) = 3e-052
Identities = 25/36 (69%), Positives = 30/36 (83%)
Frame = +1 / +3
Query: 178 SRT*LLQTS*NVTMIHL*E*M*F*DASS*TMVTNHY 285
SRT*LLQTS*N TMIHL*E*+ F*D SS* ++ ++
Sbjct: 170598 SRT*LLQTS*NATMIHL*E*IWF*DTSS*IIMVTNH 170705
Score = 44.1 bits (90), Expect(5) = 1e-065
Identities = 17/26 (65%), Positives = 19/26 (73%)
Frame = +2 / +1
Query: 467 DGRCKHGPMGREVQFNXXCXIFXQLE 544
DGRCK+G MG E+Q N C IF QLE
Sbjct: 171133 DGRCKYGSMG*EIQCNKACPIFPQLE 171210
Score = 44.1 bits (90), Expect(5) = 4e-062
Identities = 18/33 (54%), Positives = 21/33 (63%)
Frame = -3 / -3
Query: 557 VXXXLPAVXIXXXXC*IELLDP*AHAYIFHLKQ 459
+ +PAV I C IE L+P* H YIFHLKQ
Sbjct: 171223 ILAEVPAVGILDRLCCIEFLNP*THTYIFHLKQ 171125
Score = 42.8 bits (87), Expect(5) = 3e-052
Identities = 16/28 (57%), Positives = 18/28 (64%)
Frame = +1 / +3
Query: 460 CXRWKM*AWAYGSRSSIQXXXXXIXTAG 543
C RWKM* W YG R+S+Q I TAG
Sbjct: 171126 CLRWKM*VWVYGLRNSMQQSLSNIPTAG 171209
Score = 36.8 bits (74), Expect(4) = 1e-030
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = -2 / -2
Query: 555 TXXXSSCXNXXXXXLN*TSRPIGPCLHLPSXT 460
T SSC N L+* S+PI P LHLPS T
Sbjct: 171221 TGRSSSCGNIGQALLH*ISQPIDPYLHLPS*T 171126
>gb|CN127712.1|CN127712 RHOH1_24_D05.g3_A002 Acid- and alkaline-treated roots Sorghum
bicolor cDNA clone RHOH1_24_D05_A002 5', mRNA sequence
Length = 844
Score = 264 bits (571), Expect = 8e-070
Identities = 105/150 (70%), Positives = 121/150 (80%)
Frame = +3 / +2
Query: 96 FGDIVILPFIDRYELVVLKTIAICEYGVQNLTAANIMKCDDDTFVRVDVVLRRIKLNNGD 275
FGDIVILPF+DRYELVVLKT+A+C+YGVQN+TA IMKCDDDTFVR+DVVL++I N
Sbjct: 2 FGDIVILPFMDRYELVVLKTVALCQYGVQNVTADYIMKCDDDTFVRLDVVLQQIAAYNRT 181
Query: 276 KPLYMGNLNLLHRPLRTGKWAVTDEEWPEDIYPPYANGPGYVISGDIAKFIVSQRANQXX 455
PLY+GNLNL H P R+GKWAVT EEWPE YPPYANGPGYVIS DIA+ I S+ N
Sbjct: 182 LPLYLGNLNLYHSPQRSGKWAVTFEEWPEAAYPPYANGPGYVISADIARDIASRHTNHSL 361
Query: 456 XLFXMEDVSMGLWVEKFNSTXXVXYSHSWK 545
LF MEDVSMG+WVE FN++ V Y HSW+
Sbjct: 362 RLFKMEDVSMGMWVEDFNASAPVQYVHSWR 451
Score = 161 bits (346), Expect = 8e-039
Identities = 83/148 (56%), Positives = 90/148 (60%)
Frame = -1 / -2
Query: 541 QLXEYXTXXVELNFSTHRPMLTSSIXNSXXL*LARCDTMNFAMSPEIT*PGPFAYXXXXX 362
QL Y T + L STH PMLTSSI NS * D M+ AMS EIT*PGP AY
Sbjct: 447 QLWTYCTGALALKSSTHMPMLTSSILNSRRE*FVCRDAMSLAMSAEIT*PGPLAYGGYAA 268
Query: 361 XXXXXSVTAHFPVLNGLCRRLRFPIYNGLSPLFNLMRLRTTSTLTNVSSSHFMMFAAVKF 182
VTAHFP+L GL R R P Y G L + TTS+LT VSSSHFMM +AV F
Sbjct: 267 SGHSSKVTAHFPLL*GLW*RFRLPRYRGKVLL*AAICCNTTSSLTKVSSSHFMM*SAVTF 88
Query: 181 WTPYSQIAIVLRTTSS*RSINGKMTMSP 98
TPY A V RTTSS RSING+MTMSP
Sbjct: 87 CTPY*HSATVFRTTSSYRSINGRMTMSP 4
Score = 98.2 bits (208), Expect(2) = 1e-023
Identities = 45/106 (42%), Positives = 66/106 (62%)
Frame = -3 / -1
Query: 413 VTRNHIAWSVCIWRVDILRPLLVCNCPFSSPQWSVQEVKVPHI*WFVTIVQLDASQNYIY 234
V R+++A S+ + + RP L + PF + +V E++V I ++V D Q YI
Sbjct: 319 VGRDNVARSIGVRGIRRFRPFLKGDSPFPASLRTVVEIQVAQIQR*GSVVGSDLLQYYIQ 140
Query: 233 SHKCIIVTFHDVCSSQVLDPILTDSNCLKNNQLIAVYKWQNDNVSK 96
HK +++T HDV S +L P+LT NCL+N+QLI V+KWQND VSK
Sbjct: 139 PHKGVVITLHDVICSDILHPVLT*RNCLQNHQLIPVHKWQNDYVSK 2
Score = 32.2 bits (64), Expect(2) = 1e-023
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = -3 / -1
Query: 542 PAVXIXXXXC*IELLDP*AHAYIFHLKQ 459
PAV + +E+L P AHA+I HL+Q
Sbjct: 448 PAVDVLHGGARVEVLHPHAHAHILHLEQ 365
>gb|CF484299.1|CF484299 POL1_4_A06.g1_A002 Pollen Sorghum bicolor cDNA clone
POL1_4_A06_A002 5', mRNA sequence
Length = 567
Score = 261 bits (564), Expect = 7e-069
Identities = 104/158 (65%), Positives = 128/158 (81%)
Frame = +3 / +3
Query: 27 ARFFVALNSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGVQNLTAANIM 206
ARFFVAL+ R EVNV+LKKEAE+FGDIV +P++D Y+LVV+KT+AICEYGV ++A +M
Sbjct: 93 ARFFVALHGRNEVNVVLKKEAEFFGDIVFVPYLDNYDLVVMKTLAICEYGVHVVSAKYVM 272
Query: 207 KCDDDTFVRVDVVLRRIKLNNGDKPLYMGNLNLLHRPLRTGKWAVTDEEWPEDIYPPYAN 386
KCDDDTFVR+D V+ IK + LY+G++N+ HRPLR GKWAVT EEWPE+ YP YAN
Sbjct: 273 KCDDDTFVRIDSVVTEIKKVPSGRSLYLGSMNIQHRPLRQGKWAVTYEEWPEEGYPSYAN 452
Query: 387 GPGYVISGDIAKFIVSQRANQXXXLFXMEDVSMGLWVE 500
GPGYVIS DIA+FI+S+ Q LF MEDVS+GLWVE
Sbjct: 453 GPGYVISSDIAEFIMSEFMKQKLKLFKMEDVSLGLWVE 566
Score = 110 bits (234), Expect = 2e-023
Identities = 52/158 (32%), Positives = 87/158 (55%)
Frame = -3 / -2
Query: 500 LDP*AHAYIFHLKQSXXLIGTL*HDEFCYVTRNHIAWSVCIWRVDILRPLLVCNCPFSSP 321
L+P A++FH ++ L+ *HD+F + +I+ ++CI R+ + PLL+ + PF
Sbjct: 566 LNPQTQAHVFHFEELQLLLHEF*HDKFSNIR*YNISRAICI*RIPLFWPLLISHSPFPLA 387
Query: 320 QWSVQEVKVPHI*WFVTIVQLDASQNYIYSHKCIIVTFHDVCSSQVLDPILTDSNCLKNN 141
SV + P + T L+ S I SH+ II+ H + S ++PIL L NN
Sbjct: 386 *RSVLYIHTPQVKTSPTWYLLNFSDY*INSHESIIIALHYIFSRDNMNPILAYCKSLHNN 207
Query: 140 QLIAVYKWQNDNVSKVFCFFLQHYIDLLPRIQCNKESS 27
+++ + +NV K F F LQH+I L+ +QCN+++S
Sbjct: 206 KIVVIQIGHKNNVPKKFSFLLQHHIHLVSAMQCNEKTS 93
Score = 88.1 bits (186), Expect(2) = 3e-031
Identities = 46/81 (56%), Positives = 52/81 (64%)
Frame = -1 / -3
Query: 259 LMRLRTTSTLTNVSSSHFMMFAAVKFWTPYSQIAIVLRTTSS*RSINGKMTMSPKYSASF 80
L+ + T S LT VSSSHF+ + A TPYS IA V TT S* S G TMSPK SASF
Sbjct: 325 LISVTTESILTKVSSSHFITYLAETT*TPYSHIARVFITTRS*LSR*GTKTMSPKNSASF 146
Query: 79 FNITLTSFLEFSATKNRAPAY 17
F+ T TSFL SATK RA +
Sbjct: 145 FSTTFTSFLPCSATKKRATLF 83
Score = 68.0 bits (142), Expect(2) = 3e-031
Identities = 31/64 (48%), Positives = 36/64 (56%)
Frame = -1 / -3
Query: 499 STHRPMLTSSIXNSXXL*LARCDTMNFAMSPEIT*PGPFAYXXXXXXXXXXSVTAHFPVL 320
STH+P LTSSI S D +N A+S +IT PGPFAY VTAHFP
Sbjct: 565 STHKPKLTSSILKSFSFCFMNSDMINSAISDDITYPGPFAYDGYPSSGHSS*VTAHFPWR 386
Query: 319 NGLC 308
+GLC
Sbjct: 385 SGLC 374
>gb|CF488926.1|CF488926 POL1_53_C07.b1_A002 Pollen Sorghum bicolor cDNA clone
POL1_53_C07_A002 3', mRNA sequence
Length = 760
Score = 191 bits (412), Expect(2) = 7e-065
Identities = 77/127 (60%), Positives = 93/127 (73%)
Frame = +3 / +1
Query: 165 CEYGVQNLTAANIMKCDDDTFVRVDVVLRRIKLNNGDKPLYMGNLNLLHRPLRTGKWAVT 344
C V ++A +MKCDDDTFVR+D V+ IK + LYMG++N+ HRPLR GKWAVT
Sbjct: 112 CNMRVHVVSAKYVMKCDDDTFVRIDSVVTEIKKVPSGRSLYMGSMNIQHRPLRQGKWAVT 291
Query: 345 DEEWPEDIYPPYANGPGYVISGDIAKFIVSQRANQXXXLFXMEDVSMGLWVEKFNSTXXV 524
EEWPE++YP YANGPGYVIS DIA+FI+S+ Q LF MEDVS+GLWVE+FN T V
Sbjct: 292 YEEWPEEVYPSYANGPGYVISSDIAEFIMSEFMKQKLKLFKMEDVSLGLWVEQFNRTRPV 471
Query: 525 XYSHSWK 545
Y HS K
Sbjct: 472 EYIHSGK 492
Score = 77.6 bits (163), Expect(3) = 6e-025
Identities = 36/74 (48%), Positives = 41/74 (55%)
Frame = -1 / -3
Query: 529 YXTXXVELNFSTHRPMLTSSIXNSXXL*LARCDTMNFAMSPEIT*PGPFAYXXXXXXXXX 350
Y T V LN STH+P LTSSI S D +N A+S +IT PGPFAY
Sbjct: 476 YSTGLVLLNCSTHKPKLTSSILKSFSFCFMNSDMINSAISDDITYPGPFAYDGYTSSGHS 297
Query: 349 XSVTAHFPVLNGLC 308
VTAHFP +GLC
Sbjct: 296 S*VTAHFPWRSGLC 255
Score = 77.1 bits (162), Expect(2) = 7e-065
Identities = 28/42 (66%), Positives = 38/42 (90%)
Frame = +3 / +2
Query: 48 NSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEY 173
+ R EVNV+LKKEAE+FGDIV +P++D Y+LVV+KT+AICE+
Sbjct: 2 HGRNEVNVVLKKEAEFFGDIVFVPYLDNYDLVVMKTLAICEF 127
Score = 46.4 bits (95), Expect(3) = 6e-025
Identities = 25/39 (64%), Positives = 26/39 (66%)
Frame = -1 / -2
Query: 169 SQIAIVLRTTSS*RSINGKMTMSPKYSASFFNITLTSFL 53
S IA V TT S* S G TMSPK SASFF+ T TSFL
Sbjct: 123 SHIARVFITTRS*LSR*GTKTMSPKNSASFFSTTFTSFL 7
Score = 28.6 bits (56), Expect(3) = 6e-025
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = -1 / -3
Query: 259 LMRLRTTSTLTNVSSSHFMMFAA 191
L+ + T S LT VSSSHF+ + A
Sbjct: 206 LISVTTESILTKVSSSHFITYLA 138
>gb|CW486925.1|CW486925 fsbb001f252h17f0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f252h17, DNA
sequence
Length = 703
Score = 84.9 bits (179), Expect(4) = 4e-060
Identities = 32/34 (94%), Positives = 33/34 (97%)
Frame = +3 / +2
Query: 348 EEWPEDIYPPYANGPGYVISGDIAKFIVSQRANQ 449
+EWPEDIYPPYANGPGYVISGDIAKFIVSQ ANQ
Sbjct: 350 QEWPEDIYPPYANGPGYVISGDIAKFIVSQHANQ 451
Score = 80.8 bits (170), Expect(4) = 8e-055
Identities = 31/34 (91%), Positives = 32/34 (94%)
Frame = -2 / -3
Query: 450 SDWHAVTR*ILLCHQKSHSLVRLHMAGRYPQATP 349
SDWHAVTR*ILLCH+KSHSLV LHM GRYPQATP
Sbjct: 452 SDWHAVTR*ILLCHRKSHSLVHLHMVGRYPQATP 351
Score = 78.5 bits (165), Expect(4) = 1e-047
Identities = 30/34 (88%), Positives = 31/34 (91%)
Frame = -3 / -1
Query: 449 LIGTL*HDEFCYVTRNHIAWSVCIWRVDILRPLL 348
LIG L*HDEFCYVT NHIAWS+CIW VDILRPLL
Sbjct: 451 LIGML*HDEFCYVTGNHIAWSICIWWVDILRPLL 350
Score = 74.8 bits (157), Expect(4) = 4e-049
Identities = 31/34 (91%), Positives = 31/34 (91%)
Frame = +2 / +1
Query: 350 GVA*GYLPAICKRTRLCDFW*HSKIHRVTACQSE 451
GVA*GYLP ICK TRLCDF *HSKIHRVTACQSE
Sbjct: 352 GVA*GYLPTICKWTRLCDFR*HSKIHRVTACQSE 453
Score = 73.9 bits (155), Expect(4) = 4e-060
Identities = 28/29 (96%), Positives = 28/29 (96%)
Frame = +3 / +2
Query: 264 NNGDKPLYMGNLNLLHRPLRTGKWAVTDE 350
NNGDKPLYMGNLNLLHRPLRTGKWAVT E
Sbjct: 104 NNGDKPLYMGNLNLLHRPLRTGKWAVTGE 190
Score = 70.7 bits (148), Expect(4) = 8e-055
Identities = 27/31 (87%), Positives = 29/31 (93%)
Frame = -3 / -1
Query: 269 IVQLDASQNYIYSHKCIIVTFHDVCSSQVLD 177
I+QLD SQN+IYSHKCIIV FHDVCSSQVLD
Sbjct: 106 IIQLDVSQNHIYSHKCIIVAFHDVCSSQVLD 14
Score = 70.7 bits (148), Expect(4) = 4e-060
Identities = 29/31 (93%), Positives = 30/31 (96%)
Frame = +3 / +2
Query: 177 VQNLTAANIMKCDDDTFVRVDVVLRRIKLNN 269
VQNLTAANIMKCDDDTFVRVD+VLR IKLNN
Sbjct: 14 VQNLTAANIMKCDDDTFVRVDMVLRHIKLNN 106
Score = 70.3 bits (147), Expect(4) = 4e-049
Identities = 27/30 (90%), Positives = 27/30 (90%)
Frame = +2 / +1
Query: 176 GPELDCCKHHEM*R*YICESRCSSETHQVE 265
GPELDCCKHHEM R*YICESR SETHQVE
Sbjct: 13 GPELDCCKHHEMRR*YICESRYGSETHQVE 102
Score = 69.3 bits (145), Expect(4) = 8e-055
Identities = 25/29 (86%), Positives = 27/29 (93%)
Frame = -3 / -1
Query: 350 LVCNCPFSSPQWSVQEVKVPHI*WFVTIV 264
L CNCPFSS QWS+QEVKVPHI*WFVTI+
Sbjct: 190 LACNCPFSSSQWSMQEVKVPHI*WFVTII 104
Score = 69.3 bits (145), Expect(4) = 2e-039
Identities = 30/34 (88%), Positives = 31/34 (91%)
Frame = +1 / +3
Query: 349 RSGLRISTRHMQTDQAM*FLVT*QNSSCHSVPIR 450
RSGLRIST HMQ DQAM*F VT*QNSSCHS+PIR
Sbjct: 351 RSGLRISTHHMQMDQAM*FPVT*QNSSCHSMPIR 452
Score = 68.9 bits (144), Expect(4) = 1e-047
Identities = 29/31 (93%), Positives = 29/31 (93%)
Frame = -1 / -2
Query: 268 LFNLMRLRTTSTLTNVSSSHFMMFAAVKFWT 176
LFNLM LRT STLTNVSSSHFMMFAAVKFWT
Sbjct: 105 LFNLMCLRTISTLTNVSSSHFMMFAAVKFWT 13
Score = 66.1 bits (138), Expect(3) = 1e-026
Identities = 28/33 (84%), Positives = 30/33 (90%)
Frame = -2 / -3
Query: 273 HHCST*CVSELHLLSQMYHRHIS*CLQQSSSGP 175
H+ ST*CVSE +LLSQMYHR IS*CLQQSSSGP
Sbjct: 110 HYYST*CVSEPYLLSQMYHRRIS*CLQQSSSGP 12
Score = 64.8 bits (135), Expect(4) = 4e-049
Identities = 25/28 (89%), Positives = 25/28 (89%)
Frame = +1 / +3
Query: 268 MVTNHYIWGTLTSCTDH*GLENGQLQTR 351
MVTNHYIWGTLTSC DH* LENGQLQ R
Sbjct: 108 MVTNHYIWGTLTSCIDH*ELENGQLQAR 191
Score = 61.6 bits (128), Expect(4) = 4e-060
Identities = 24/33 (72%), Positives = 25/33 (75%)
Frame = +3 / +2
Query: 459 LFXMEDVSMGLWVEKFNSTXXVXYSHSWKXXXY 557
LF MEDVSMGLWVEKFN+T V YSHSW Y
Sbjct: 542 LFKMEDVSMGLWVEKFNATKPVQYSHSWNFCQY 640
Score = 59.7 bits (124), Expect(4) = 2e-039
Identities = 24/28 (85%), Positives = 25/28 (89%)
Frame = +2 / +1
Query: 269 W*QTIIYGEP*PPAQTIEDWKMGSYRRG 352
W*QTIIYGEP*P A T E+WKMGSYRRG
Sbjct: 109 W*QTIIYGEP*PLA*TTENWKMGSYRRG 192
Score = 57.4 bits (119), Expect(4) = 1e-047
Identities = 24/28 (85%), Positives = 25/28 (89%)
Frame = -2 / -3
Query: 351 PRL*LPIFQSSMVCAGG*GSPYIMVCHH 268
PRL*LPIFQ S+V A G*GSPYIMVCHH
Sbjct: 191 PRL*LPIFQFSVVYARG*GSPYIMVCHH 108
Score = 57.4 bits (119), Expect(3) = 1e-026
Identities = 24/25 (96%), Positives = 24/25 (96%)
Frame = -1 / -2
Query: 451 L*LARCDTMNFAMSPEIT*PGPFAY 377
L*LA CDTMNFAMSPEIT*PGPFAY
Sbjct: 453 L*LACCDTMNFAMSPEIT*PGPFAY 379
Score = 52.4 bits (108), Expect(4) = 8e-055
Identities = 23/33 (69%), Positives = 24/33 (72%)
Frame = -1 / -2
Query: 556 YXXXFQLXEYXTXXVELNFSTHRPMLTSSIXNS 458
Y FQL EY T V LNFSTHRP+LTSSI NS
Sbjct: 639 YWQKFQLWEYWTGFVALNFSTHRPILTSSILNS 541
Score = 48.7 bits (100), Expect(4) = 2e-039
Identities = 25/36 (69%), Positives = 30/36 (83%)
Frame = +1 / +3
Query: 178 SRT*LLQTS*NVTMIHL*E*M*F*DASS*TMVTNHY 285
SRT*LLQTS*N TMIHL*E*+ F*D SS* ++ ++
Sbjct: 15 SRT*LLQTS*NATMIHL*E*IWF*DTSS*IIMVTNH 122
Score = 44.1 bits (90), Expect(4) = 4e-049
Identities = 17/26 (65%), Positives = 19/26 (73%)
Frame = +2 / +1
Query: 467 DGRCKHGPMGREVQFNXXCXIFXQLE 544
DGRCK+G MG E+Q N C IF QLE
Sbjct: 550 DGRCKYGSMG*EIQCNKACPIFPQLE 627
Score = 44.1 bits (90), Expect(4) = 1e-047
Identities = 18/33 (54%), Positives = 21/33 (63%)
Frame = -3 / -1
Query: 557 VXXXLPAVXIXXXXC*IELLDP*AHAYIFHLKQ 459
+ +PAV I C IE L+P* H YIFHLKQ
Sbjct: 640 ILAEVPAVGILDRLCCIEFLNP*THTYIFHLKQ 542
Score = 42.8 bits (87), Expect(4) = 2e-039
Identities = 16/28 (57%), Positives = 18/28 (64%)
Frame = +1 / +3
Query: 460 CXRWKM*AWAYGSRSSIQXXXXXIXTAG 543
C RWKM* W YG R+S+Q I TAG
Sbjct: 543 CLRWKM*VWVYGLRNSMQQSLSNIPTAG 626
Score = 36.8 bits (74), Expect(3) = 1e-026
Identities = 17/32 (53%), Positives = 19/32 (59%)
Frame = -2 / -3
Query: 555 TXXXSSCXNXXXXXLN*TSRPIGPCLHLPSXT 460
T SSC N L+* S+PI P LHLPS T
Sbjct: 638 TGRSSSCGNIGQALLH*ISQPIDPYLHLPS*T 543
>gb|CW173784.1|CW173784 104_585_11157034_148_36551_037 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11157034, DNA
sequence
Length = 683
Score = 80.3 bits (169), Expect(3) = 9e-046
Identities = 30/33 (90%), Positives = 32/33 (96%)
Frame = +3 / +1
Query: 348 EEWPEDIYPPYANGPGYVISGDIAKFIVSQRAN 446
+EWPEDIYPPYANGPGYVISGDIAKFIVSQ A+
Sbjct: 556 QEWPEDIYPPYANGPGYVISGDIAKFIVSQHAH 654
Score = 75.3 bits (158), Expect(3) = 6e-043
Identities = 28/33 (84%), Positives = 30/33 (90%)
Frame = -3 / -3
Query: 446 IGTL*HDEFCYVTRNHIAWSVCIWRVDILRPLL 348
+G L*HDEFCYVT NHIAWS+CIW VDILRPLL
Sbjct: 654 MGML*HDEFCYVTGNHIAWSICIWWVDILRPLL 556
Score = 74.8 bits (157), Expect(3) = 1e-038
Identities = 30/34 (88%), Positives = 31/34 (91%)
Frame = -2 / -2
Query: 450 SDWHAVTR*ILLCHQKSHSLVRLHMAGRYPQATP 349
SD HAVTR*ILLCH+KSHSLV LHM GRYPQATP
Sbjct: 658 SDGHAVTR*ILLCHRKSHSLVHLHMVGRYPQATP 557
Score = 73.9 bits (155), Expect(3) = 9e-046
Identities = 28/29 (96%), Positives = 28/29 (96%)
Frame = +3 / +1
Query: 264 NNGDKPLYMGNLNLLHRPLRTGKWAVTDE 350
NNGDKPLYMGNLNLLHRPLRTGKWAVT E
Sbjct: 310 NNGDKPLYMGNLNLLHRPLRTGKWAVTGE 396
Score = 72.1 bits (151), Expect(3) = 2e-040
Identities = 30/34 (88%), Positives = 30/34 (88%)
Frame = +2 / +3
Query: 350 GVA*GYLPAICKRTRLCDFW*HSKIHRVTACQSE 451
GVA*GYLP ICK TRLCDF *HSKIHRVTAC SE
Sbjct: 558 GVA*GYLPTICKWTRLCDFR*HSKIHRVTACPSE 659
Score = 70.7 bits (148), Expect(3) = 6e-043
Identities = 27/31 (87%), Positives = 29/31 (93%)
Frame = -3 / -3
Query: 269 IVQLDASQNYIYSHKCIIVTFHDVCSSQVLD 177
I+QLD SQN+IYSHKCIIV FHDVCSSQVLD
Sbjct: 312 IIQLDVSQNHIYSHKCIIVAFHDVCSSQVLD 220
Score = 70.7 bits (148), Expect(3) = 9e-046
Identities = 29/31 (93%), Positives = 30/31 (96%)
Frame = +3 / +1
Query: 177 VQNLTAANIMKCDDDTFVRVDVVLRRIKLNN 269
VQNLTAANIMKCDDDTFVRVD+VLR IKLNN
Sbjct: 220 VQNLTAANIMKCDDDTFVRVDMVLRHIKLNN 312
Score = 70.3 bits (147), Expect(3) = 2e-040
Identities = 27/30 (90%), Positives = 27/30 (90%)
Frame = +2 / +3
Query: 176 GPELDCCKHHEM*R*YICESRCSSETHQVE 265
GPELDCCKHHEM R*YICESR SETHQVE
Sbjct: 219 GPELDCCKHHEMRR*YICESRYGSETHQVE 308
Score = 69.3 bits (145), Expect(3) = 8e-032
Identities = 30/34 (88%), Positives = 31/34 (91%)
Frame = +1 / +2
Query: 349 RSGLRISTRHMQTDQAM*FLVT*QNSSCHSVPIR 450
RSGLRIST HMQ DQAM*F VT*QNSSCHS+PIR
Sbjct: 557 RSGLRISTHHMQMDQAM*FPVT*QNSSCHSMPIR 658
Score = 69.3 bits (145), Expect(3) = 6e-043
Identities = 25/29 (86%), Positives = 27/29 (93%)
Frame = -3 / -3
Query: 350 LVCNCPFSSPQWSVQEVKVPHI*WFVTIV 264
L CNCPFSS QWS+QEVKVPHI*WFVTI+
Sbjct: 396 LACNCPFSSSQWSMQEVKVPHI*WFVTII 310
Score = 68.9 bits (144), Expect(3) = 1e-038
Identities = 29/31 (93%), Positives = 29/31 (93%)
Frame = -1 / -1
Query: 268 LFNLMRLRTTSTLTNVSSSHFMMFAAVKFWT 176
LFNLM LRT STLTNVSSSHFMMFAAVKFWT
Sbjct: 311 LFNLMCLRTISTLTNVSSSHFMMFAAVKFWT 219
Score = 64.8 bits (135), Expect(3) = 2e-040
Identities = 25/28 (89%), Positives = 25/28 (89%)
Frame = +1 / +2
Query: 268 MVTNHYIWGTLTSCTDH*GLENGQLQTR 351
MVTNHYIWGTLTSC DH* LENGQLQ R
Sbjct: 314 MVTNHYIWGTLTSCIDH*ELENGQLQAR 397
Score = 59.7 bits (124), Expect(3) = 8e-032
Identities = 24/28 (85%), Positives = 25/28 (89%)
Frame = +2 / +3
Query: 269 W*QTIIYGEP*PPAQTIEDWKMGSYRRG 352
W*QTIIYGEP*P A T E+WKMGSYRRG
Sbjct: 315 W*QTIIYGEP*PLA*TTENWKMGSYRRG 398
Score = 57.4 bits (119), Expect(3) = 1e-038
Identities = 24/28 (85%), Positives = 25/28 (89%)
Frame = -2 / -2
Query: 351 PRL*LPIFQSSMVCAGG*GSPYIMVCHH 268
PRL*LPIFQ S+V A G*GSPYIMVCHH
Sbjct: 397 PRL*LPIFQFSVVYARG*GSPYIMVCHH 314
Score = 48.7 bits (100), Expect(3) = 8e-032
Identities = 25/36 (69%), Positives = 30/36 (83%)
Frame = +1 / +2
Query: 178 SRT*LLQTS*NVTMIHL*E*M*F*DASS*TMVTNHY 285
SRT*LLQTS*N TMIHL*E*+ F*D SS* ++ ++
Sbjct: 221 SRT*LLQTS*NATMIHL*E*IWF*DTSS*IIMVTNH 328
>gb|CW098046.1|CW098046 104_464_11002794_148_34367_073 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11002794, DNA
sequence
Length = 660
Score = 84.9 bits (179), Expect(3) = 3e-035
Identities = 32/34 (94%), Positives = 33/34 (97%)
Frame = +3 / -2
Query: 348 EEWPEDIYPPYANGPGYVISGDIAKFIVSQRANQ 449
+EWPEDIYPPYANGPGYVISGDIAKFIVSQ ANQ
Sbjct: 449 QEWPEDIYPPYANGPGYVISGDIAKFIVSQHANQ 348
Score = 80.8 bits (170), Expect(3) = 9e-031
Identities = 31/34 (91%), Positives = 32/34 (94%)
Frame = -2 / +2
Query: 450 SDWHAVTR*ILLCHQKSHSLVRLHMAGRYPQATP 349
SDWHAVTR*ILLCH+KSHSLV LHM GRYPQATP
Sbjct: 347 SDWHAVTR*ILLCHRKSHSLVHLHMVGRYPQATP 448
Score = 78.5 bits (165), Expect(3) = 3e-026
Identities = 30/34 (88%), Positives = 31/34 (91%)
Frame = -3 / +3
Query: 449 LIGTL*HDEFCYVTRNHIAWSVCIWRVDILRPLL 348
LIG L*HDEFCYVT NHIAWS+CIW VDILRPLL
Sbjct: 348 LIGML*HDEFCYVTGNHIAWSICIWWVDILRPLL 449
Score = 74.8 bits (157), Expect(3) = 8e-025
Identities = 31/34 (91%), Positives = 31/34 (91%)
Frame = +2 / -1
Query: 350 GVA*GYLPAICKRTRLCDFW*HSKIHRVTACQSE 451
GVA*GYLP ICK TRLCDF *HSKIHRVTACQSE
Sbjct: 447 GVA*GYLPTICKWTRLCDFR*HSKIHRVTACQSE 346
Score = 69.3 bits (145), Expect(3) = 1e-022
Identities = 30/34 (88%), Positives = 31/34 (91%)
Frame = +1 / -3
Query: 349 RSGLRISTRHMQTDQAM*FLVT*QNSSCHSVPIR 450
RSGLRIST HMQ DQAM*F VT*QNSSCHS+PIR
Sbjct: 448 RSGLRISTHHMQMDQAM*FPVT*QNSSCHSMPIR 347
Score = 61.6 bits (128), Expect(3) = 3e-035
Identities = 24/33 (72%), Positives = 25/33 (75%)
Frame = +3 / -2
Query: 459 LFXMEDVSMGLWVEKFNSTXXVXYSHSWKXXXY 557
LF MEDVSMGLWVEKFN+T V YSHSW Y
Sbjct: 257 LFKMEDVSMGLWVEKFNATKPVQYSHSWNFCQY 159
Score = 52.4 bits (108), Expect(3) = 9e-031
Identities = 23/33 (69%), Positives = 24/33 (72%)
Frame = -1 / +1
Query: 556 YXXXFQLXEYXTXXVELNFSTHRPMLTSSIXNS 458
Y FQL EY T V LNFSTHRP+LTSSI NS
Sbjct: 160 YWQKFQLWEYWTGFVALNFSTHRPILTSSILNS 258
Score = 44.1 bits (90), Expect(3) = 8e-025
Identities = 17/26 (65%), Positives = 19/26 (73%)
Frame = +2 / -1
Query: 467 DGRCKHGPMGREVQFNXXCXIFXQLE 544
DGRCK+G MG E+Q N C IF QLE
Sbjct: 249 DGRCKYGSMG*EIQCNKACPIFPQLE 172
Score = 44.1 bits (90), Expect(3) = 3e-026
Identities = 18/33 (54%), Positives = 21/33 (63%)
Frame = -3 / +3
Query: 557 VXXXLPAVXIXXXXC*IELLDP*AHAYIFHLKQ 459
+ +PAV I C IE L+P* H YIFHLKQ
Sbjct: 159 ILAEVPAVGILDRLCCIEFLNP*THTYIFHLKQ 257
Score = 42.8 bits (87), Expect(3) = 1e-022
Identities = 16/28 (57%), Positives = 18/28 (64%)
Frame = +1 / -3
Query: 460 CXRWKM*AWAYGSRSSIQXXXXXIXTAG 543
C RWKM* W YG R+S+Q I TAG
Sbjct: 256 CLRWKM*VWVYGLRNSMQQSLSNIPTAG 173
Score = 42.8 bits (87), Expect(3) = 3e-035
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = +3 / -2
Query: 300 NLLHRPLRTGKWAVTDE 350
NLLHRPLRTGKWAVT E
Sbjct: 659 NLLHRPLRTGKWAVTGE 609
Score = 40.9 bits (83), Expect(3) = 9e-031
Identities = 14/17 (82%), Positives = 15/17 (88%)
Frame = -3 / +3
Query: 350 LVCNCPFSSPQWSVQEV 300
L CNCPFSS QWS+QEV
Sbjct: 609 LACNCPFSSSQWSMQEV 659
Score = 36.3 bits (73), Expect(3) = 3e-026
Identities = 13/15 (86%), Positives = 15/15 (100%)
Frame = -1 / +1
Query: 343 VTAHFPVLNGLCRRL 299
VTAHFPVL+GLC+RL
Sbjct: 616 VTAHFPVLSGLCKRL 660
Score = 35.0 bits (70), Expect(3) = 8e-025
Identities = 14/17 (82%), Positives = 14/17 (82%)
Frame = +1 / -3
Query: 301 TSCTDH*GLENGQLQTR 351
TSC DH* LENGQLQ R
Sbjct: 658 TSCIDH*ELENGQLQAR 608
Score = 34.5 bits (69), Expect(3) = 1e-022
Identities = 14/18 (77%), Positives = 15/18 (83%)
Frame = +2 / -1
Query: 299 *PPAQTIEDWKMGSYRRG 352
*P A T E+WKMGSYRRG
Sbjct: 660 *PLA*TTENWKMGSYRRG 607
>gb|CW042123.1|CW042123 104_276_10506948_114_30476 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10506948, DNA
sequence
Length = 711
Score = 83.5 bits (176), Expect(2) = 4e-034
Identities = 31/44 (70%), Positives = 40/44 (90%)
Frame = +3 / -3
Query: 48 NSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGV 179
+ R EVNV+LKKEAE+FGDIV +P++D Y+LVV+KT+AICEYGV
Sbjct: 508 HGRNEVNVVLKKEAEFFGDIVFVPYLDNYDLVVMKTLAICEYGV 377
Score = 82.2 bits (173), Expect(2) = 4e-034
Identities = 33/58 (56%), Positives = 42/58 (72%)
Frame = +3 / -1
Query: 177 VQNLTAANIMKCDDDTFVRVDVVLRRIKLNNGDKPLYMGNLNLLHRPLRTGKWAVTDE 350
V ++A +MKCDDDTFVR+D V+ IK + LYMG++N+ HRPLR GKWAVT E
Sbjct: 279 VHVVSAKYVMKCDDDTFVRIDSVVTEIKKVPSGRSLYMGSMNIQHRPLRQGKWAVTYE 106
>gb|CW437404.1|CW437404 fsbb001f154i03k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f154i03, DNA
sequence
Length = 710
Score = 83.5 bits (176), Expect(2) = 4e-034
Identities = 31/44 (70%), Positives = 40/44 (90%)
Frame = +3 / -1
Query: 48 NSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGV 179
+ R EVNV+LKKEAE+FGDIV +P++D Y+LVV+KT+AICEYGV
Sbjct: 473 HGRNEVNVVLKKEAEFFGDIVFVPYLDNYDLVVMKTLAICEYGV 342
Score = 82.2 bits (173), Expect(2) = 4e-034
Identities = 33/58 (56%), Positives = 42/58 (72%)
Frame = +3 / -2
Query: 177 VQNLTAANIMKCDDDTFVRVDVVLRRIKLNNGDKPLYMGNLNLLHRPLRTGKWAVTDE 350
V ++A +MKCDDDTFVR+D V+ IK + LYMG++N+ HRPLR GKWAVT E
Sbjct: 244 VHVVSAKYVMKCDDDTFVRIDSVVTEIKKVPSGRSLYMGSMNIQHRPLRQGKWAVTYE 71
>gb|CW111980.1|CW111980 104_485_11104632_116_34538_086 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11104632, DNA
sequence
Length = 621
Score = 83.5 bits (176), Expect(2) = 4e-034
Identities = 31/44 (70%), Positives = 40/44 (90%)
Frame = +3 / -2
Query: 48 NSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGV 179
+ R EVNV+LKKEAE+FGDIV +P++D Y+LVV+KT+AICEYGV
Sbjct: 440 HGRNEVNVVLKKEAEFFGDIVFVPYLDNYDLVVMKTLAICEYGV 309
Score = 82.2 bits (173), Expect(2) = 4e-034
Identities = 33/58 (56%), Positives = 42/58 (72%)
Frame = +3 / -3
Query: 177 VQNLTAANIMKCDDDTFVRVDVVLRRIKLNNGDKPLYMGNLNLLHRPLRTGKWAVTDE 350
V ++A +MKCDDDTFVR+D V+ IK + LYMG++N+ HRPLR GKWAVT E
Sbjct: 211 VHVVSAKYVMKCDDDTFVRIDSVVTEIKKVPSGRSLYMGSMNIQHRPLRQGKWAVTYE 38
>gb|CW209770.1|CW209770 104_640_11189431_116_37430_032 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11189431, DNA
sequence
Length = 666
Score = 86.3 bits (182), Expect(2) = 1e-033
Identities = 35/44 (79%), Positives = 40/44 (90%)
Frame = +3 / -2
Query: 48 NSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGV 179
+ R EVNV LKKEAE+FGDIVI+PF+D Y+LVVLKTIAICEYGV
Sbjct: 608 HGRNEVNVELKKEAEFFGDIVIVPFMDSYDLVVLKTIAICEYGV 477
Score = 77.6 bits (163), Expect(2) = 1e-033
Identities = 32/58 (55%), Positives = 40/58 (68%)
Frame = +3 / -3
Query: 177 VQNLTAANIMKCDDDTFVRVDVVLRRIKLNNGDKPLYMGNLNLLHRPLRTGKWAVTDE 350
V ++A IMKCDDDTFVR+D V+ +K LY+GN+N H+PLR GKWAVT E
Sbjct: 391 VHVVSARYIMKCDDDTFVRLDSVMAEVKKIQNGISLYIGNMNYHHKPLRDGKWAVTYE 218
>gb|BG159208.1|BG159208 RHIZ2_27_F09.b1_A003 Rhizome2 (RHIZ2) Sorghum propinquum cDNA, mRNA
sequence
Length = 548
Score = 129 bits (277), Expect = 3e-029
Identities = 54/74 (72%), Positives = 63/74 (85%)
Frame = +3 / +3
Query: 27 ARFFVALNSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGVQNLTAANIM 206
ARFFVALN +KEVN LKKEAE+F DIVI+PFID Y+LVVLKT+AI EYGV+ + A +M
Sbjct: 321 ARFFVALNGKKEVNAELKKEAEFFQDIVIVPFIDSYDLVVLKTVAIAEYGVRVVPAKYVM 500
Query: 207 KCDDDTFVRVDVVL 248
KCDDDTFVR+D VL
Sbjct: 501 KCDDDTFVRIDSVL 542
>gb|CF489000.1|CF489000 POL1_53_C07.g1_A002 Pollen Sorghum bicolor cDNA clone
POL1_53_C07_A002 5', mRNA sequence
Length = 669
Score = 92.3 bits (195), Expect(2) = 6e-029
Identities = 35/49 (71%), Positives = 45/49 (91%)
Frame = +3 / +3
Query: 27 ARFFVALNSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEY 173
ARFFVAL+ R EVNV+LKKEAE+FGDIV +P++D Y+LVV+KT+AICE+
Sbjct: 393 ARFFVALHGRNEVNVVLKKEAEFFGDIVFVPYLDNYDLVVMKTLAICEF 539
Score = 56.1 bits (116), Expect(2) = 6e-029
Identities = 22/47 (46%), Positives = 31/47 (65%)
Frame = +3 / +2
Query: 165 CEYGVQNLTAANIMKCDDDTFVRVDVVLRRIKLNNGDKPLYMGNLNL 305
C V ++A +MKCDDDTFVR+D V+ IK + LYMG++N+
Sbjct: 524 CNMRVHVVSAKYVMKCDDDTFVRIDSVVTEIKKVPSGRSLYMGSMNI 664
>gb|CW486926.1|CW486926 fsbb001f252h17k0 Sorghum methylation filtered library (LibID: 104)
Sorghum bicolor genomic clone fsbb001f252h17, DNA
sequence
Length = 660
Score = 84.9 bits (179), Expect(2) = 2e-028
Identities = 32/34 (94%), Positives = 33/34 (97%)
Frame = +3 / -1
Query: 348 EEWPEDIYPPYANGPGYVISGDIAKFIVSQRANQ 449
+EWPEDIYPPYANGPGYVISGDIAKFIVSQ ANQ
Sbjct: 618 QEWPEDIYPPYANGPGYVISGDIAKFIVSQHANQ 517
Score = 80.8 bits (170), Expect(2) = 2e-024
Identities = 31/34 (91%), Positives = 32/34 (94%)
Frame = -2 / +3
Query: 450 SDWHAVTR*ILLCHQKSHSLVRLHMAGRYPQATP 349
SDWHAVTR*ILLCH+KSHSLV LHM GRYPQATP
Sbjct: 516 SDWHAVTR*ILLCHRKSHSLVHLHMVGRYPQATP 617
Score = 78.5 bits (165), Expect(2) = 3e-021
Identities = 30/34 (88%), Positives = 31/34 (91%)
Frame = -3 / +1
Query: 449 LIGTL*HDEFCYVTRNHIAWSVCIWRVDILRPLL 348
LIG L*HDEFCYVT NHIAWS+CIW VDILRPLL
Sbjct: 517 LIGML*HDEFCYVTGNHIAWSICIWWVDILRPLL 618
Score = 61.6 bits (128), Expect(2) = 2e-028
Identities = 24/33 (72%), Positives = 25/33 (75%)
Frame = +3 / -1
Query: 459 LFXMEDVSMGLWVEKFNSTXXVXYSHSWKXXXY 557
LF MEDVSMGLWVEKFN+T V YSHSW Y
Sbjct: 426 LFKMEDVSMGLWVEKFNATKPVQYSHSWNFCQY 328
Score = 52.4 bits (108), Expect(2) = 2e-024
Identities = 23/33 (69%), Positives = 24/33 (72%)
Frame = -1 / +2
Query: 556 YXXXFQLXEYXTXXVELNFSTHRPMLTSSIXNS 458
Y FQL EY T V LNFSTHRP+LTSSI NS
Sbjct: 329 YWQKFQLWEYWTGFVALNFSTHRPILTSSILNS 427
Score = 44.1 bits (90), Expect(2) = 3e-021
Identities = 18/33 (54%), Positives = 21/33 (63%)
Frame = -3 / +1
Query: 557 VXXXLPAVXIXXXXC*IELLDP*AHAYIFHLKQ 459
+ +PAV I C IE L+P* H YIFHLKQ
Sbjct: 328 ILAEVPAVGILDRLCCIEFLNP*THTYIFHLKQ 426
>gb|BI098036.1|BI098036 IP1_31_C06.g1_A002 Immature pannicle 1 (IP1) Sorghum bicolor cDNA,
mRNA sequence
Length = 676
Score = 121 bits (258), Expect = 1e-026
Identities = 49/62 (79%), Positives = 50/62 (80%)
Frame = +3 / +2
Query: 372 PPYANGPGYVISGDIAKFIVSQRANQXXXLFXMEDVSMGLWVEKFNSTXXVXYSHSWKXX 551
PPYANGPGYVISGDIAKFIVSQ ANQ LF MEDVSMGLWVEKFN+T V YSHSW
Sbjct: 2 PPYANGPGYVISGDIAKFIVSQHANQSLRLFKMEDVSMGLWVEKFNATKPVQYSHSWNFC 181
Query: 552 XY 557
Y
Sbjct: 182 QY 187
Score = 105 bits (225), Expect = 4e-022
Identities = 47/60 (78%), Positives = 48/60 (80%)
Frame = -1 / -2
Query: 556 YXXXFQLXEYXTXXVELNFSTHRPMLTSSIXNSXXL*LARCDTMNFAMSPEIT*PGPFAY 377
Y FQL EY T V LNFSTHRP+LTSSI NS L*LA CDTMNFAMSPEIT*PGPFAY
Sbjct: 186 YWQKFQLWEYWTGFVALNFSTHRPILTSSILNSLKL*LACCDTMNFAMSPEIT*PGPFAY 7
Score = 103 bits (220), Expect = 2e-021
Identities = 41/61 (67%), Positives = 45/61 (73%)
Frame = -3 / -1
Query: 557 VXXXLPAVXIXXXXC*IELLDP*AHAYIFHLKQSXXLIGTL*HDEFCYVTRNHIAWSVCI 378
+ +PAV I C IE L+P* H YIFHLKQS LIG L*HDEFCYVT NHIAWS+CI
Sbjct: 187 ILAEVPAVGILDRLCCIEFLNP*THTYIFHLKQS*TLIGML*HDEFCYVTGNHIAWSICI 8
Query: 377 W 375
W
Sbjct: 7 W 5
Score = 102 bits (218), Expect = 4e-021
Identities = 43/58 (74%), Positives = 45/58 (77%)
Frame = +2 / +1
Query: 371 PAICKRTRLCDFW*HSKIHRVTACQSEXXTVXDGRCKHGPMGREVQFNXXCXIFXQLE 544
P ICK TRLCDF *HSKIHRVTACQSE TV DGRCK+G MG E+Q N C IF QLE
Sbjct: 1 PTICKWTRLCDFR*HSKIHRVTACQSEFKTV*DGRCKYGSMG*EIQCNKACPIFPQLE 174
>gb|CW044337.1|CW044337 104_280_10508891_115_30242 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 10508891, DNA
sequence
Length = 561
Score = 105 bits (224), Expect(2) = 4e-023
Identities = 41/47 (87%), Positives = 42/47 (89%)
Frame = +2 / +1
Query: 47 EFEEGGQCNVEERSRILWRHCHFAIYRPL*AGCS*DNCYL*VWGPEL 187
EF+E GQCN EERSRILWRHCHFAIYR L*AGCS*DNCYL*VWG L
Sbjct: 85 EFKERGQCNAEERSRILWRHCHFAIYRSL*AGCS*DNCYL*VWGKTL 225
Score = 105 bits (224), Expect(2) = 8e-026
Identities = 44/46 (95%), Positives = 46/46 (100%)
Frame = +3 / +2
Query: 48 NSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGVQN 185
NSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGV++
Sbjct: 86 NSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGVRH 223
Score = 104 bits (221), Expect(2) = 8e-024
Identities = 39/41 (95%), Positives = 40/41 (97%)
Frame = -3 / -3
Query: 176 PILTDSNCLKNNQLIAVYKWQNDNVSKVFCFFLQHYIDLLP 54
PILTDSNCLKNNQLIA+YKWQNDNVSKVFCFFLQHYIDL P
Sbjct: 214 PILTDSNCLKNNQLIAIYKWQNDNVSKVFCFFLQHYIDLFP 92
Score = 98.7 bits (209), Expect(2) = 5e-022
Identities = 44/47 (93%), Positives = 45/47 (95%)
Frame = -1 / -1
Query: 187 KFWTPYSQIAIVLRTTSS*RSINGKMTMSPKYSASFFNITLTSFLEF 47
K TPYSQIAIVLRTTSS*RSINGKMTMSPKYSASFF+ITLTSFLEF
Sbjct: 225 KCLTPYSQIAIVLRTTSS*RSINGKMTMSPKYSASFFSITLTSFLEF 85
Score = 33.6 bits (67), Expect(2) = 8e-026
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +2 / +3
Query: 176 GPELDCCKHH 205
GPELDCCKHH
Sbjct: 531 GPELDCCKHH 560
Score = 28.1 bits (55), Expect(2) = 8e-024
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = -1 / -2
Query: 205 MMFAAVKFWT 176
MMFAAVKFWT
Sbjct: 560 MMFAAVKFWT 531
Score = 27.6 bits (54), Expect(2) = 5e-022
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = -3 / -1
Query: 206 HDVCSSQVLD 177
HDVCSSQVLD
Sbjct: 561 HDVCSSQVLD 532
Score = 24.4 bits (47), Expect(2) = 4e-023
Identities = 10/10 (100%), Positives = 10/10 (100%)
Frame = +3 / +1
Query: 177 VQNLTAANIM 206
VQNLTAANIM
Sbjct: 532 VQNLTAANIM 561
>gb|CF484228.1|CF484228 POL1_4_A06.b1_A002 Pollen Sorghum bicolor cDNA clone
POL1_4_A06_A002 3', mRNA sequence
Length = 661
Score = 111 bits (238), Expect = 7e-024
Identities = 44/66 (66%), Positives = 51/66 (77%)
Frame = +3 / +2
Query: 348 EEWPEDIYPPYANGPGYVISGDIAKFIVSQRANQXXXLFXMEDVSMGLWVEKFNSTXXVX 527
EEWPE++YP YANGPGYVIS DIA+FI+S+ Q LF MEDVS+GLWVE+FN T V
Sbjct: 2 EEWPEEVYPSYANGPGYVISSDIAEFIMSEFMKQKLKLFKMEDVSLGLWVEQFNRTRPVE 181
Query: 528 YSHSWK 545
Y HS K
Sbjct: 182 YIHSGK 199
>gb|BF656435.1|BF656435 FM1_45_G12.g2_A003 Floral-Induced Meristem 1 (FM1) Sorghum
propinquum cDNA, mRNA sequence
Length = 633
Score = 109 bits (233), Expect = 3e-023
Identities = 45/58 (77%), Positives = 46/58 (79%)
Frame = +3 / +1
Query: 384 NGPGYVISGDIAKFIVSQRANQXXXLFXMEDVSMGLWVEKFNSTXXVXYSHSWKXXXY 557
NGPGYVISGDIAKFIVSQ ANQ LF MEDVSMGLWVEKFN+T V YSHSW Y
Sbjct: 1 NGPGYVISGDIAKFIVSQHANQSLRLFKMEDVSMGLWVEKFNATKPVQYSHSWNFCQY 174
>gb|CW241144.1|CW241144 104_700_11218605_148_37542_093 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11218605, DNA
sequence
Length = 718
Score = 86.8 bits (183), Expect(2) = 1e-022
Identities = 36/47 (76%), Positives = 41/47 (87%)
Frame = +3 / -3
Query: 48 NSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGVQNL 188
N R EVN LKKEA+YFGDIVI+PF+D Y+LVVLKTIAI EYGV+NL
Sbjct: 311 NGRNEVNEELKKEADYFGDIVIVPFMDSYDLVVLKTIAIVEYGVRNL 171
Score = 40.5 bits (82), Expect(2) = 1e-022
Identities = 15/29 (51%), Positives = 23/29 (79%)
Frame = +3 / -1
Query: 171 YGVQNLTAANIMKCDDDTFVRVDVVLRRI 257
+ V+ + A +IMKCDDDTFVR++ VL ++
Sbjct: 88 FQVRVIPAKHIMKCDDDTFVRIESVLDQV 2
>gb|CL176804.1|CL176804 104_383_10893374_116_31773_206 Sorghum methylation-filtered library
(LibID: 104) Sorghum bicolor genomic clone 10893374, DNA
sequence
Length = 426
Score = 79.9 bits (168), Expect(2) = 4e-022
Identities = 32/58 (55%), Positives = 41/58 (70%)
Frame = +3 / -1
Query: 177 VQNLTAANIMKCDDDTFVRVDVVLRRIKLNNGDKPLYMGNLNLLHRPLRTGKWAVTDE 350
V ++A +MKCDDDTFVR+D V+ IK + LYMG++N+ HRP R GKWAVT E
Sbjct: 261 VHVVSAKYVMKCDDDTFVRIDSVVTEIKKVPSGRSLYMGSMNIQHRPXRQGKWAVTYE 88
Score = 45.5 bits (93), Expect(2) = 4e-022
Identities = 15/22 (68%), Positives = 21/22 (95%)
Frame = +3 / -3
Query: 114 LPFIDRYELVVLKTIAICEYGV 179
+P++D Y+LVV+KT+AICEYGV
Sbjct: 424 VPYLDNYDLVVMKTLAICEYGV 359
>gb|CW098045.1|CW098045 104_464_11002794_116_34363_073 Sorghum methylation filtered library
(LibID: 104) Sorghum bicolor genomic clone 11002794, DNA
sequence
Length = 593
Score = 105 bits (224), Expect = 6e-022
Identities = 41/47 (87%), Positives = 42/47 (89%)
Frame = +2 / +2
Query: 47 EFEEGGQCNVEERSRILWRHCHFAIYRPL*AGCS*DNCYL*VWGPEL 187
EF+E GQCN EERSRILWRHCHFAIYR L*AGCS*DNCYL*VWG L
Sbjct: 314 EFKERGQCNAEERSRILWRHCHFAIYRSL*AGCS*DNCYL*VWGKTL 454
Score = 105 bits (224), Expect = 6e-022
Identities = 44/46 (95%), Positives = 46/46 (100%)
Frame = +3 / +3
Query: 48 NSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGVQN 185
NSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGV++
Sbjct: 315 NSRKEVNVMLKKEAEYFGDIVILPFIDRYELVVLKTIAICEYGVRH 452
Score = 104 bits (221), Expect = 1e-021
Identities = 39/41 (95%), Positives = 40/41 (97%)
Frame = -3 / -1
Query: 176 PILTDSNCLKNNQLIAVYKWQNDNVSKVFCFFLQHYIDLLP 54
PILTDSNCLKNNQLIA+YKWQNDNVSKVFCFFLQHYIDL P
Sbjct: 443 PILTDSNCLKNNQLIAIYKWQNDNVSKVFCFFLQHYIDLFP 321
Database: Sorghum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:54 PM
Number of letters in database: 491,359,669
Number of sequences in database: 832,831
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 689,868,365
Number of Sequences: 832831
Number of extensions: 9980386
Number of successful extensions: 79614
Number of sequences better than 1.0e-020: 24
length of database: 163,786,556
effective HSP length: 53
effective length of database: 119,646,513
effective search space used: 16391572281
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)