TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3748516.2.1
(1155 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved l... 107 3e-036
gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Med... 100 8e-035
gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula c... 109 3e-032
gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula... 107 7e-032
gb|BF648216.1|BF648216 NF045D01EC1F1012 Elicited cell cultu... 80 3e-025
gb|BG448066.1|BG448066 NF105C08EC1F1056 Elicited cell cultu... 77 4e-022
gb|AW687498.2|AW687498 NF010C04RT1F1033 Developing root Med... 75 5e-021
gb|AC148226.7| Medicago truncatula clone mth2-5i20, complet... 50 6e-021
>gb|BG455193.1|BG455193 NF100G04PL1F1034 Phosphate starved leaf Medicago truncatula cDNA
clone NF100G04PL 5', mRNA sequence
Length = 654
Score = 107 bits (229), Expect(4) = 3e-036
Identities = 43/100 (43%), Positives = 60/100 (60%)
Frame = -3 / +2
Query: 673 PVAKYHFDLSGTAFGSMASWGLNDKLRHAGIIDMQFRRVPCNFPGLTITFRVQHGSNPMY 494
P + HFDLSG AFG MA G N +LR+ G I + +RR C + G I F+V GS P +
Sbjct: 191 PSDRTHFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFW 370
Query: 493 LAVLIEHENKDGDVVQADIMQSNSGHWEPMHESWGSIWRI 374
L++L+E E+ DGD+ I ++ S W M+ WG+ W I
Sbjct: 371 LSLLVEFEDGDGDISSMHIQEAGSSEWLQMNHLWGANWNI 490
Score = 42.8 bits (87), Expect(4) = 3e-036
Identities = 14/28 (50%), Positives = 24/28 (85%)
Frame = -3 / +2
Query: 361 PLRGPFSMRITNESGRQLVAKNVIPANY 278
PLRGPFS++++ +G+ L AK+VIP+++
Sbjct: 500 PLRGPFSVKLSTSTGKSLTAKDVIPSHW 583
Score = 35.9 bits (72), Expect(4) = 3e-036
Identities = 9/17 (52%), Positives = 15/17 (88%)
Frame = -3 / +2
Query: 790 IFKDGKGCGSCYQIRCL 740
++K G+GCG CY+++CL
Sbjct: 77 LYKKGEGCGECYKVKCL 127
Score = 23.5 bits (45), Expect(4) = 3e-036
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -3 / +2
Query: 733 NHPACSGVAQTVIITD 686
+H CS A TVIITD
Sbjct: 128 DHTICSKRAVTVIITD 175
>gb|BG453428.1|BG453428 NF091G11LF1F1085 Developing leaf Medicago truncatula cDNA clone
NF091G11LF 5', mRNA sequence
Length = 638
Score = 100 bits (214), Expect(3) = 8e-035
Identities = 42/80 (52%), Positives = 58/80 (72%)
Frame = -3 / +3
Query: 667 AKYHFDLSGTAFGSMASWGLNDKLRHAGIIDMQFRRVPCNFPGLTITFRVQHGSNPMYLA 488
A+YHFDLSG+AFGSMA G +++LR+AG I ++ RRV CN+ G TI F V GSN Y A
Sbjct: 18 AEYHFDLSGSAFGSMAVSGEDEELRNAGKIVIEHRRVQCNYGGSTIAFHVDSGSNQEYFA 197
Query: 487 VLIEHENKDGDVVQADIMQS 428
LIE+E DGD+ + ++ ++
Sbjct: 198 ALIEYEEGDGDLNKVELKEA 257
Score = 49.6 bits (102), Expect(3) = 8e-035
Identities = 14/33 (42%), Positives = 23/33 (69%)
Frame = -3 / +3
Query: 427 NSGHWEPMHESWGSIWRIDPNRPLRGPFSMRIT 329
+S W+ M +SWG++W+ D PL+ P S+R+T
Sbjct: 261 DSSSWDTMQQSWGAVWKFDKGAPLKAPISIRLT 359
Score = 36.8 bits (74), Expect(3) = 8e-035
Identities = 16/31 (51%), Positives = 21/31 (67%)
Frame = -3 / +3
Query: 331 TNESGRQLVAKNVIPANYIPDVDYRSFVQY* 239
T +SG+ +VA NVIPA + P YRS V +*
Sbjct: 360 TLKSGKTIVAHNVIPAGWKPGQTYRSIVNF* 452
>gb|CA917715.1|CA917715 EST641862 GPOD Medicago truncatula cDNA clone GPOD-37E9, mRNA
sequence
Length = 769
Score = 109 bits (233), Expect(4) = 3e-032
Identities = 43/100 (43%), Positives = 61/100 (61%)
Frame = -3 / +2
Query: 673 PVAKYHFDLSGTAFGSMASWGLNDKLRHAGIIDMQFRRVPCNFPGLTITFRVQHGSNPMY 494
P + HFDLSG AFG MA G N +LR+ G I + +RR C + G I F+V GS P +
Sbjct: 443 PSDRTHFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFW 622
Query: 493 LAVLIEHENKDGDVVQADIMQSNSGHWEPMHESWGSIWRI 374
L++L+E E+ DGD+ I ++ S W M+ WG+IW +
Sbjct: 623 LSLLVEFEDGDGDISSMHIQEAGSSEWLQMNHLWGAIWNM 742
Score = 33.6 bits (67), Expect(4) = 3e-032
Identities = 8/17 (47%), Positives = 14/17 (82%)
Frame = -3 / +2
Query: 790 IFKDGKGCGSCYQIRCL 740
++ G+GCG CY+++CL
Sbjct: 329 LYNKGEGCGECYKVKCL 379
Score = 29.5 bits (58), Expect(4) = 3e-032
Identities = 10/27 (37%), Positives = 13/27 (48%)
Frame = -3 / +2
Query: 955 QDRSLLSYSSGWLPAKATWYGAPTGAG 875
Q + + + W P ATWYG G G
Sbjct: 167 QSKHSVHFDPHWHPGTATWYGEAEGDG 247
Score = 23.5 bits (45), Expect(4) = 3e-032
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -3 / +2
Query: 733 NHPACSGVAQTVIITD 686
+H CS A TVIITD
Sbjct: 380 DHTICSKRAVTVIITD 427
>gb|AL379968.1|AL379968 MtBB48E05F1 MtBB Medicago truncatula cDNA clone MtBB48E05 T3, mRNA
sequence
Length = 476
Score = 107 bits (229), Expect(4) = 7e-032
Identities = 43/100 (43%), Positives = 60/100 (60%)
Frame = -3 / +2
Query: 673 PVAKYHFDLSGTAFGSMASWGLNDKLRHAGIIDMQFRRVPCNFPGLTITFRVQHGSNPMY 494
P + HFDLSG AFG MA G N +LR+ G I + +RR C + G I F+V GS P +
Sbjct: 119 PSDRTHFDLSGAAFGHMAVHGENGQLRNRGEIQVLYRRTTCVYAGKNIAFQVNEGSTPFW 298
Query: 493 LAVLIEHENKDGDVVQADIMQSNSGHWEPMHESWGSIWRI 374
L++L+E E+ DGD+ I ++ S W M+ WG+ W I
Sbjct: 299 LSLLVEFEDGDGDISSMHIQEAGSSEWLQMNHLWGANWNI 418
Score = 35.9 bits (72), Expect(4) = 7e-032
Identities = 9/17 (52%), Positives = 15/17 (88%)
Frame = -3 / +2
Query: 790 IFKDGKGCGSCYQIRCL 740
++K G+GCG CY+++CL
Sbjct: 5 LYKKGEGCGECYKVKCL 55
Score = 27.6 bits (54), Expect(4) = 7e-032
Identities = 8/16 (50%), Positives = 14/16 (87%)
Frame = -3 / +2
Query: 361 PLRGPFSMRITNESGR 314
PLRGPFS++++ +G+
Sbjct: 428 PLRGPFSVKLSTSTGK 475
Score = 23.5 bits (45), Expect(4) = 7e-032
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -3 / +2
Query: 733 NHPACSGVAQTVIITD 686
+H CS A TVIITD
Sbjct: 56 DHTICSKRAVTVIITD 103
>gb|BF648216.1|BF648216 NF045D01EC1F1012 Elicited cell culture Medicago truncatula cDNA
clone NF045D01EC 5', mRNA sequence
Length = 596
Score = 80.3 bits (169), Expect(3) = 3e-025
Identities = 30/75 (40%), Positives = 45/75 (60%)
Frame = -3 / +3
Query: 598 LRHAGIIDMQFRRVPCNFPGLTITFRVQHGSNPMYLAVLIEHENKDGDVVQADIMQSNSG 419
LR+ G I + +RR PC +PG I F V GS P +L++L+E E+ +GD+ I ++ S
Sbjct: 54 LRNRGQIPVIYRRTPCKYPGKKIAFHVNEGSTPFWLSLLVEFEDAEGDIGTMHIRENGSS 233
Query: 418 HWEPMHESWGSIWRI 374
W M+ WG+ W I
Sbjct: 234 EWLQMNHLWGANWCI 278
Score = 52.4 bits (108), Expect(3) = 3e-025
Identities = 17/40 (42%), Positives = 31/40 (77%)
Frame = -3 / +3
Query: 361 PLRGPFSMRITNESGRQLVAKNVIPANYIPDVDYRSFVQY 242
PLRGPFS+++++ +GR L A++V+P+N++P Y S + +
Sbjct: 288 PLRGPFSVKLSSSTGRTLSARDVVPSNWVPKATYTSRLNF 407
Score = 22.1 bits (42), Expect(3) = 3e-025
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = -3 / +2
Query: 649 LSGTAFGSMASWGLN 605
LSG AFG MA G N
Sbjct: 2 LSGAAFGHMAISGEN 46
>gb|BG448066.1|BG448066 NF105C08EC1F1056 Elicited cell culture Medicago truncatula cDNA
clone NF105C08EC 5', mRNA sequence
Length = 652
Score = 77.1 bits (162), Expect(3) = 4e-022
Identities = 32/64 (50%), Positives = 40/64 (62%)
Frame = -3 / +1
Query: 673 PVAKYHFDLSGTAFGSMASWGLNDKLRHAGIIDMQFRRVPCNFPGLTITFRVQHGSNPMY 494
P HFDLSG AFG MA G N +LR+ G I + +RR PC +PG I F V GS P +
Sbjct: 460 PTDTTHFDLSGAAFGHMAISGENGQLRNRGQIPVIYRRTPCKYPGKKIAFHVNEGSTPFW 639
Query: 493 LAVL 482
L++L
Sbjct: 640 LSLL 651
Score = 35.4 bits (71), Expect(3) = 4e-022
Identities = 9/20 (45%), Positives = 17/20 (85%)
Frame = -3 / +1
Query: 790 IFKDGKGCGSCYQIRCLKSN 731
+F G+GCG+CY+++CL ++
Sbjct: 346 LFMKGEGCGACYKVKCLDNS 405
Score = 31.8 bits (63), Expect(3) = 4e-022
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -3 / +1
Query: 922 WLPAKATWYGAPTGAG 875
W P ATWYG P G G
Sbjct: 217 WYPGTATWYGDPEGDG 264
>gb|AW687498.2|AW687498 NF010C04RT1F1033 Developing root Medicago truncatula cDNA clone
NF010C04RT 5', mRNA sequence
Length = 522
Score = 75.3 bits (158), Expect(2) = 5e-021
Identities = 28/76 (36%), Positives = 45/76 (59%)
Frame = -3 / +2
Query: 601 KLRHAGIIDMQFRRVPCNFPGLTITFRVQHGSNPMYLAVLIEHENKDGDVVQADIMQSNS 422
+LR+ G I + +RR C + G + F+V GS P +L++L+E E+ DGD+ I ++ S
Sbjct: 116 QLRNRGEIPVLYRRTTCVYAGKNLAFQVNEGSTPYWLSLLVEFEDGDGDISSMYIQEAGS 295
Query: 421 GHWEPMHESWGSIWRI 374
W M+ WG+ W I
Sbjct: 296 SEWLQMNHLWGANWNI 343
Score = 47.8 bits (98), Expect(2) = 5e-021
Identities = 16/40 (40%), Positives = 28/40 (70%)
Frame = -3 / +2
Query: 361 PLRGPFSMRITNESGRQLVAKNVIPANYIPDVDYRSFVQY 242
PLRGPFS++++ +G+ + K+VIP+N+ P Y S + +
Sbjct: 353 PLRGPFSVKLSTSTGKSITVKDVIPSNWSPKSTYTSRLNF 472
>gb|AC148226.7| Medicago truncatula clone mth2-5i20, complete sequence
Length = 84259
Score = 50.1 bits (103), Expect(5) = 6e-021
Identities = 19/42 (45%), Positives = 29/42 (69%)
Frame = -3 / +1
Query: 541 GLTITFRVQHGSNPMYLAVLIEHENKDGDVVQADIMQSNSGH 416
G +I F + +G+NP A IE+EN +GD+VQ ++ QSN+ H
Sbjct: 65848 GNSIVFTIDNGANPYCFATEIEYENGNGDLVQVELSQSNTWH 65973
Score = 46.4 bits (95), Expect(5) = 6e-021
Identities = 19/33 (57%), Positives = 26/33 (78%)
Frame = -3 / +1
Query: 658 HFDLSGTAFGSMASWGLNDKLRHAGIIDMQFRR 560
HFDLSGTAFGS+A+ D LR+AG I++++ R
Sbjct: 65611 HFDLSGTAFGSLANPNQADNLRNAGKINIRYTR 65709
Score = 42.3 bits (86), Expect(5) = 6e-021
Identities = 14/29 (48%), Positives = 18/29 (62%)
Frame = -3 / +2
Query: 826 PFSSMTSCGNEPIFKDGKGCGSCYQIRCL 740
P S M S G IF G+GCG+CYQ+ +
Sbjct: 64031 PLSKMISAGGPSIFLGGRGCGACYQVHAM 64117
Score = 34.5 bits (69), Expect(5) = 6e-021
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = -3 / +1
Query: 922 WLPAKATWYGAPTGAG 875
W A ATWYGAP GAG
Sbjct: 63682 WQTAIATWYGAPNGAG 63729
Score = 33.6 bits (67), Expect(5) = 6e-021
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -3 / +3
Query: 328 NESGRQLVAKNVIPANYIPDVDYRSFVQY* 239
N S ++A+NVIP N+ P YRS V +*
Sbjct: 70653 NGSNNTIIAENVIPQNWQPGQVYRSLVNF* 70742
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 785,217,764
Number of Sequences: 392609
Number of extensions: 9530062
Number of successful extensions: 77700
Number of sequences better than 1.0e-020: 12
length of database: 147,244,331
effective HSP length: 55
effective length of database: 125,650,836
effective search space used: 41339125044
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)