TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2655972.2.1
(666 letters)
Database: Mt_nucl_with_EST.fasta
392,609 sequences; 441,732,993 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AC148995.2| Medicago truncatula chromosome 7 BAC clone m... 208 9e-053
gb|CX530987.1|CX530987 s13dNF20C02MJ007_248115 Methyl Jasmo... 201 8e-051
gb|AW685614.1|AW685614 NF029F03NR1F1000 Nodulated root Medi... 187 1e-046
gb|CX531592.1|CX531592 s13dNF29D10MJ079_257130 Methyl Jasmo... 160 1e-038
>gb|AC148995.2| Medicago truncatula chromosome 7 BAC clone mth2-49l23, complete
sequence
Length = 128239
Score = 208 bits (448), Expect = 9e-053
Identities = 78/127 (61%), Positives = 100/127 (78%)
Frame = +2 / -2
Query: 32 SPVLFFFGCRNQENDFLYKDFWLTHAQDEGVLSSKRGGGFFVAFSRDQPQKVYVQPKIKE 211
+P++FFFGC N++ DFLYKDFWL H+Q+ GVLS GGGF+VAFSRDQP+KVYVQ K++E
Sbjct: 93471 APIIFFFGCWNEDGDFLYKDFWLNHSQNNGVLSESTGGGFYVAFSRDQPEKVYVQHKLRE 93292
Query: 212 QSSRLWNLLLSDAVIYIAGSSTKMPADVTAALEEVICKEGGVKQADASKWLRDLERAGRL 391
S R+WNLL A +YIAGS TKMP DVT+A EE++ KE V + DA +W+R LE+ G+
Sbjct: 93291 HSGRVWNLLAEGASVYIAGSLTKMPTDVTSAFEEIVSKENDVSKEDAVRWIRALEKCGKY 93112
Query: 392 NIETWS* 412
+IE WS*
Sbjct: 93111 HIEAWS* 93091
Score = 133 bits (285), Expect = 3e-030
Identities = 69/125 (55%), Positives = 79/125 (63%)
Frame = -1 / +2
Query: 408 DHVSMLSLPALSRSLSHFEASACLTPPSLQITSSSAAVTSAGIFVEEPAM*ITASDSNKF 229
DH SM LP S +L H AS+ T SL SS A VTS GI V EPA+* A +NKF
Sbjct: 93095 DHASM*YLPHFSNALIHLTASSFETSFSLDTISSKADVTSVGILVNEPAI*TEAPSANKF 93274
Query: 228 HSLELCSFILGCT*TFCG*SREKATKKPPPLFEDNTPSSCA*VSQKSLYRKSFSWFLQPK 49
+L LCS L T*TF G S EKAT KPPP+ ++TP C * +QKSLY+KS S F QPK
Sbjct: 93275 QTLPLCSLNLC*T*TFSGWSLEKAT*KPPPVDSESTPLFCE*FNQKSLYKKSPSSFQQPK 93454
Query: 48 KNRTG 34
KN G
Sbjct: 93455 KNIIG 93469
>gb|CX530987.1|CX530987 s13dNF20C02MJ007_248115 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 580
Score = 201 bits (434), Expect = 8e-051
Identities = 78/127 (61%), Positives = 98/127 (77%)
Frame = +2 / -1
Query: 32 SPVLFFFGCRNQENDFLYKDFWLTHAQDEGVLSSKRGGGFFVAFSRDQPQKVYVQPKIKE 211
+P++FFFGC N++ DFLYKD WL H+Q+ GVLS GGGF+VAFSRDQ +KVYVQ K+KE
Sbjct: 577 APIMFFFGCWNEDGDFLYKDLWLNHSQNNGVLSEANGGGFYVAFSRDQLEKVYVQHKMKE 398
Query: 212 QSSRLWNLLLSDAVIYIAGSSTKMPADVTAALEEVICKEGGVKQADASKWLRDLERAGRL 391
S R+WNLL A +YIAGSSTKM DVT+A EE++ KE V + DA +W+R LER G+
Sbjct: 397 HSGRVWNLLADGAAVYIAGSSTKMLTDVTSAFEEIVSKENEVSKEDAVRWIRALERCGKY 218
Query: 392 NIETWS* 412
+IE WS*
Sbjct: 217 HIEAWS* 197
Score = 138 bits (296), Expect = 8e-032
Identities = 70/128 (54%), Positives = 82/128 (64%)
Frame = -1 / +3
Query: 417 HNYDHVSMLSLPALSRSLSHFEASACLTPPSLQITSSSAAVTSAGIFVEEPAM*ITASDS 238
++ DH SM LP LS +L H AS+ T SL SS A VTS I V+EPA+* A +
Sbjct: 192 NSQDHASM*YLPHLSNALIHLTASSFETSFSLDTISSKADVTSVSILVDEPAI*TAAPSA 371
Query: 237 NKFHSLELCSFILGCT*TFCG*SREKATKKPPPLFEDNTPSSCA*VSQKSLYRKSFSWFL 58
NKF +L LCSFIL T*TF SREKAT KPPP ++TP C * + KSLY+KS S F
Sbjct: 372 NKFQTLPLCSFILC*T*TFSSWSREKAT*KPPPFASESTPLFCE*FNHKSLYKKSPSSFQ 551
Query: 57 QPKKNRTG 34
QPKKN G
Sbjct: 552 QPKKNIIG 575
>gb|AW685614.1|AW685614 NF029F03NR1F1000 Nodulated root Medicago truncatula cDNA clone
NF029F03NR 5', mRNA sequence
Length = 666
Score = 187 bits (403), Expect = 1e-046
Identities = 70/112 (62%), Positives = 88/112 (78%)
Frame = +2 / +3
Query: 26 TXSPVLFFFGCRNQENDFLYKDFWLTHAQDEGVLSSKRGGGFFVAFSRDQPQKVYVQPKI 205
+ +P++FFFGC N++ DFLYKD WL H+Q+ GVLS GGGF+VAFSRDQP+KVYVQ K+
Sbjct: 321 STAPIMFFFGCWNEDGDFLYKDLWLNHSQNNGVLSEANGGGFYVAFSRDQPEKVYVQHKM 500
Query: 206 KEQSSRLWNLLLSDAVIYIAGSSTKMPADVTAALEEVICKEGGVKQADASKW 361
KE S R+WNLL A +YIAGSSTKMP DVT+A EE++ KE V + DA +W
Sbjct: 501 KEHSGRVWNLLADGAAVYIAGSSTKMPTDVTSAFEEIVSKENEVSKEDAVRW 656
Score = 125 bits (268), Expect = 6e-028
Identities = 62/111 (55%), Positives = 73/111 (65%)
Frame = -1 / -3
Query: 366 LSHFEASACLTPPSLQITSSSAAVTSAGIFVEEPAM*ITASDSNKFHSLELCSFILGCT* 187
+ H AS+ T SL SS A VTS GI V+EPA+* A +N+F +L LCSFIL T*
Sbjct: 661 IPHLTASSFETSFSLDTISSKADVTSVGILVDEPAI*TAAPSANRFQTLPLCSFILC*T* 482
Query: 186 TFCG*SREKATKKPPPLFEDNTPSSCA*VSQKSLYRKSFSWFLQPKKNRTG 34
TF G SREKAT KPPP ++TP C * + KSLY+KS S F QPKKN G
Sbjct: 481 TFSGWSREKAT*KPPPFASESTPLFCE*FNHKSLYKKSPSSFQQPKKNIIG 329
>gb|CX531592.1|CX531592 s13dNF29D10MJ079_257130 Methyl Jasmonate-Elicited Root Cell
Suspension Culture Medicago truncatula cDNA, mRNA
sequence
Length = 609
Score = 160 bits (345), Expect = 1e-038
Identities = 60/90 (66%), Positives = 73/90 (81%)
Frame = +2 / +3
Query: 26 TXSPVLFFFGCRNQENDFLYKDFWLTHAQDEGVLSSKRGGGFFVAFSRDQPQKVYVQPKI 205
+ +P++FFFGC N++ DFLYKD WL H+Q+ GVLS GGGF+VAFSRDQP+KVYVQ K+
Sbjct: 339 STAPIMFFFGCWNEDGDFLYKDLWLNHSQNNGVLSEANGGGFYVAFSRDQPEKVYVQHKM 518
Query: 206 KEQSSRLWNLLLSDAVIYIAGSSTKMPADV 295
KE S R+WNLL A +YIAGSSTKMP DV
Sbjct: 519 KEHSGRVWNLLXDGAAVYIAGSSTKMPTDV 608
Score = 110 bits (235), Expect = 2e-023
Identities = 52/87 (59%), Positives = 60/87 (68%)
Frame = -1 / -3
Query: 294 TSAGIFVEEPAM*ITASDSNKFHSLELCSFILGCT*TFCG*SREKATKKPPPLFEDNTPS 115
TS GI V+EPA+* A N+F +L LCSFIL T*TF G SREKAT KPPP ++TP
Sbjct: 607 TSVGILVDEPAI*TAAPSXNRFQTLPLCSFILC*T*TFSGWSREKAT*KPPPFASESTPL 428
Query: 114 SCA*VSQKSLYRKSFSWFLQPKKNRTG 34
C * + KSLY+KS S F QPKKN G
Sbjct: 427 FCE*FNHKSLYKKSPSSFQQPKKNIIG 347
Database: Mt_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:25 PM
Number of letters in database: 441,732,993
Number of sequences in database: 392,609
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 695,554,964
Number of Sequences: 392609
Number of extensions: 10073746
Number of successful extensions: 46204
Number of sequences better than 1.0e-020: 5
length of database: 147,244,331
effective HSP length: 54
effective length of database: 126,043,445
effective search space used: 21049255315
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)