TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3713002.2.1
(923 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY804253.1| Medicago sativa acidic glucanase (Glu) mRNA,... 195 6e-051
gb|U27179.1|MSU27179 Medicago sativa acidic glucanase mRNA,... 107 2e-024
>gb|AY804253.1| Medicago sativa acidic glucanase (Glu) mRNA, complete cds
Length = 1113
Score = 195 bits (421), Expect = 6e-051
Identities = 77/145 (53%), Positives = 100/145 (68%)
Frame = +3 / +1
Query: 9 GSPLMANIYPYLAWAYNPSAMDMSYALFTSSGTVVQDGAYGYQNLFDTTVDAFYVAMGKN 188
G+PL+ N+YPY + NP + + YALFTS G +VQDG GYQNLFD +D+ + A+
Sbjct: 607 GAPLLVNVYPYFSHVGNPRDISLPYALFTSPGVMVQDGPNGYQNLFDAMLDSVHAALDNT 786
Query: 189 GGSGVPLVVSESGWPSGGGVQATPANARVYNQYLINHVGRGTPRHPGAIETYLFSMFNEN 368
G V +VVSESGWPS GG + NAR+Y LI HVG+GTPR P A ETY+F+MF+EN
Sbjct: 787 GIGWVNVVVSESGWPSDGGAATSYDNARIYLDNLIRHVGKGTPRRPWATETYIFAMFDEN 966
Query: 369 QKESGVEQNWGLFYPNMQHVYPISF 443
QK +E+++G+FYPN Q YP F
Sbjct: 967 QKSPELEKHFGVFYPNKQKKYPFGF 1041
Score = 76.7 bits (161), Expect(2) = 8e-024
Identities = 38/87 (43%), Positives = 45/87 (51%)
Frame = -2 / -2
Query: 268 ALAGVAWTPPPDGHPLSDTTSGTPEPPFFPMAT*KASTVVSKRFWYP*APSCTTVPEEVK 89
AL+ PP +G PLSDTT+ T P A S + S +FWYP PSCT P EVK
Sbjct: 866 ALSYEVAAPPSEGQPLSDTTTFTQPIPVLSRAACTESNIASNKFWYPFGPSCTITPGEVK 687
Query: 88 SA*LMSMALGLYAQARYG*MLAMSGDP 8
A* M+ GL + G* L G P
Sbjct: 686 RA*GRDMSRGLPTWLK*G*TLTSKGAP 606
Score = 48.3 bits (99), Expect(2) = 8e-024
Identities = 22/41 (53%), Positives = 27/41 (65%)
Frame = -2 / -2
Query: 421 CMLG*KSPQFCSTPLSFWFSLNMEKR*VSMAPGWRGVPRPT 299
C+LG* +P+ S FWFS N+ K *VS+A G GVP PT
Sbjct: 1019 CLLG*NTPKCFSNSGLFWFSSNIAKI*VSVAQGLLGVPLPT 897
>gb|U27179.1|MSU27179 Medicago sativa acidic glucanase mRNA, complete cds
Length = 1374
Score = 107 bits (228), Expect = 2e-024
Identities = 42/79 (53%), Positives = 54/79 (68%)
Frame = +3 / +1
Query: 9 GSPLMANIYPYLAWAYNPSAMDMSYALFTSSGTVVQDGAYGYQNLFDTTVDAFYVAMGKN 188
G+PL+ N+YPY + NP + + YALFTS G +VQDG GYQNLFD +D+ + A+
Sbjct: 658 GAPLLVNVYPYFSHVGNPRDISLPYALFTSPGVMVQDGPNGYQNLFDAMLDSVHAALDNT 837
Query: 189 GGSGVPLVVSESGWPSGGG 245
G V +VVSESGWPS GG
Sbjct: 838 GIGWVNVVVSESGWPSDGG 894
Score = 76.2 bits (160), Expect(2) = 4e-023
Identities = 36/79 (45%), Positives = 42/79 (53%)
Frame = -2 / -2
Query: 244 PPPDGHPLSDTTSGTPEPPFFPMAT*KASTVVSKRFWYP*APSCTTVPEEVKSA*LMSMA 65
PP +G PLSDTT+ T P A S + S +FWYP PSCT P EVK A* M+
Sbjct: 893 PPSEGQPLSDTTTFTQPIPVLSRAACTESNIASNKFWYPFGPSCTITPGEVKRA*GRDMS 714
Query: 64 LGLYAQARYG*MLAMSGDP 8
GL + G* L G P
Sbjct: 713 RGLPTWLK*G*TLTSKGAP 657
Score = 46.4 bits (95), Expect(2) = 4e-023
Identities = 21/41 (51%), Positives = 27/41 (65%)
Frame = -2 / -2
Query: 421 CMLG*KSPQFCSTPLSFWFSLNMEKR*VSMAPGWRGVPRPT 299
C+LG* +P+ S FWFS N+ K *VS+A G GVP P+
Sbjct: 1067 CLLG*NTPKCFSNSGLFWFSSNIAKI*VSVAQGLLGVPLPS 945
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,133,141
Number of Sequences: 7669
Number of extensions: 71745
Number of successful extensions: 760
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 242563464
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)