TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1805320.2.1
(955 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF135596.1|AF135596 Medicago sativa Skp1 (SKP1) mRNA, pa... 158 4e-061
gb|CO513371.1|CO513371 s13dSG38C0400022_129906 Glandular tr... 136 4e-054
gb|CO515179.1|CO515179 s13dSG62C1100086_399223 Glandular tr... 117 4e-048
gb|CO513276.1|CO513276 s13dSG37B0100009_129716 Glandular tr... 100 1e-043
gb|CO517166.1|CO517166 s13dSG33D0700062_474416 Glandular tr... 149 7e-037
>gb|AF135596.1|AF135596 Medicago sativa Skp1 (SKP1) mRNA, partial cds
Length = 592
Score = 158 bits (339), Expect(2) = 4e-061
Identities = 66/81 (81%), Positives = 72/81 (88%)
Frame = -2 / +1
Query: 579 EDLKNWDADFVKVDQATLFDLILAANYLNIKGLLDLTCQTVADMIKGKTPEEIRKTFNIK 400
++LK+WD++FVKVDQATLFDLILAANYLNIK LLDLTCQTVADMIKGKTPEEIRKTFNIK
Sbjct: 220 DELKSWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNIK 399
Query: 399 NDFTPXXXXXXXXENQWAFE* 337
NDF+P ENQWAFE*
Sbjct: 400 NDFSPEEEEEVRRENQWAFE* 462
Score = 127 bits (273), Expect(2) = 4e-044
Identities = 62/78 (79%), Positives = 66/78 (84%)
Frame = +2 / -3
Query: 338 YSKAHWFSLRISSSSSGVKSFLMLKVLRISSGVLPLIMSATV*QVRSSRPLMFR*FAAKM 517
+S AHWFSLR SSSSSG KSFLML VLRISSGV PLIMSATV QVRSS+ L+F+* AAKM
Sbjct: 461 HSNAHWFSLRTSSSSSGEKSFLMLNVLRISSGVFPLIMSATVWQVRSSKLLIFK*LAAKM 282
Query: 518 RSKSVAWSTLTKSASQFL 571
RSKSVA STLT S SQ L
Sbjct: 281 RSKSVA*STLTNSESQDL 228
Score = 96.4 bits (204), Expect(2) = 3e-026
Identities = 36/60 (60%), Positives = 51/60 (85%)
Frame = +1 / -2
Query: 385 RGEVILDVEGLANLFWCLALDHVSNSLTGQVEQTLDVQIVCSQDEVEKRGLVDLDEVRIP 564
R E+IL+VE LA+L WCL+LDHV NSL G+++Q LD+Q++ SQDE++KR L++LDE+ IP
Sbjct: 414 R*EIILNVECLADLLWCLSLDHVCNSLAGKIQQALDIQVISSQDEIKKRSLINLDELGIP 235
Score = 91.8 bits (194), Expect(2) = 4e-061
Identities = 39/56 (69%), Positives = 43/56 (76%)
Frame = -2 / +1
Query: 846 KMITLKSSDGXXXXXXXXXXXESQTIRHMIEDDCADNGIPLPNVNSKILSKVIEYC 679
+ ITLKSSDG ESQTI+HMIEDDCAD+GIPLPNV SKIL+KVIEYC
Sbjct: 7 RKITLKSSDGETFEVDEAVALESQTIKHMIEDDCADSGIPLPNVTSKILAKVIEYC 174
Score = 65.2 bits (136), Expect(2) = 4e-044
Identities = 30/55 (54%), Positives = 35/55 (63%)
Frame = +2 / -3
Query: 677 LQYSITLDRILELTLGRGMPLSAQSSSIMWRMVCDXXXXXXXXXXXXXXELLRVI 841
LQYSIT +IL +TLGRG+PLSAQSSSI+ +VCD EL RVI
Sbjct: 176 LQYSITFAKILLVTLGRGIPLSAQSSSIICLIVCDSKATASSTSKVSPSELFRVI 12
Score = 36.8 bits (74), Expect(2) = 3e-026
Identities = 15/31 (48%), Positives = 22/31 (70%)
Frame = +1 / -2
Query: 679 AVLDYLG*DLGVDVRKRDAVVGAVILDHVAD 771
A+L++L DL +V KRD+ V A+I DH+ D
Sbjct: 174 AILNHLRQDLAGNVGKRDSTVSAIIFDHMLD 82
>gb|CO513371.1|CO513371 s13dSG38C0400022_129906 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 547
Score = 136 bits (292), Expect(2) = 4e-054
Identities = 55/65 (84%), Positives = 62/65 (95%)
Frame = -2 / +1
Query: 579 EDLKNWDADFVKVDQATLFDLILAANYLNIKGLLDLTCQTVADMIKGKTPEEIRKTFNIK 400
++LK+WD++FVKVDQATLFDLILAANYLNIK LLDLTCQTVADMIKGKTPEE +TFNIK
Sbjct: 352 DELKSWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEENPQTFNIK 531
Query: 399 NDFTP 385
NDF+P
Sbjct: 532 NDFSP 546
Score = 94.5 bits (200), Expect(2) = 4e-034
Identities = 36/60 (60%), Positives = 49/60 (81%)
Frame = +1 / -2
Query: 385 RGEVILDVEGLANLFWCLALDHVSNSLTGQVEQTLDVQIVCSQDEVEKRGLVDLDEVRIP 564
R E+ILDVE L L WCL+LDHV NSL G+++Q LD+Q++ SQDE++KR L++LDE+ IP
Sbjct: 546 R*EIILDVECLRILLWCLSLDHVCNSLAGKIQQALDIQVISSQDEIKKRSLINLDELGIP 367
Score = 90.0 bits (190), Expect(2) = 4e-054
Identities = 38/56 (67%), Positives = 42/56 (75%)
Frame = -2 / +1
Query: 846 KMITLKSSDGXXXXXXXXXXXESQTIRHMIEDDCADNGIPLPNVNSKILSKVIEYC 679
+ ITLKSSDG ESQTI+HMIEDDC D+GIPLPNV SKIL+KVIEYC
Sbjct: 139 RKITLKSSDGETFEVDEAVALESQTIKHMIEDDCVDSGIPLPNVTSKILAKVIEYC 306
Score = 90.0 bits (190), Expect(2) = 2e-024
Identities = 46/62 (74%), Positives = 49/62 (79%)
Frame = +2 / -3
Query: 386 GVKSFLMLKVLRISSGVLPLIMSATV*QVRSSRPLMFR*FAAKMRSKSVAWSTLTKSASQ 565
G KSFLML V SSGV PLIMSATV QVRSS+ L+F+* AAKMRSKSVA STLT S SQ
Sbjct: 545 GEKSFLMLNVCGFSSGVFPLIMSATVWQVRSSKLLIFK*LAAKMRSKSVA*STLTNSESQ 366
Query: 566 FL 571
L
Sbjct: 365 DL 360
Score = 65.2 bits (136), Expect(2) = 4e-034
Identities = 30/55 (54%), Positives = 35/55 (63%)
Frame = +2 / -3
Query: 677 LQYSITLDRILELTLGRGMPLSAQSSSIMWRMVCDXXXXXXXXXXXXXXELLRVI 841
LQYSIT +IL +TLGRG+PLS QSSSI+ +VCD ELLRVI
Sbjct: 308 LQYSITFAKILLVTLGRGIPLSTQSSSIICLIVCDSNATASSTSKVSPSELLRVI 144
Score = 36.8 bits (74), Expect(2) = 2e-024
Identities = 15/31 (48%), Positives = 22/31 (70%)
Frame = +1 / -2
Query: 679 AVLDYLG*DLGVDVRKRDAVVGAVILDHVAD 771
A+L++L DL +V KRD+ V A+I DH+ D
Sbjct: 306 AILNHLRQDLAGNVGKRDSTVNAIIFDHMLD 214
>gb|CO515179.1|CO515179 s13dSG62C1100086_399223 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 503
Score = 117 bits (251), Expect(2) = 4e-048
Identities = 47/63 (74%), Positives = 54/63 (85%)
Frame = -2 / +3
Query: 573 LKNWDADFVKVDQATLFDLILAANYLNIKGLLDLTCQTVADMIKGKTPEEIRKTFNIKND 394
LKNWDADFVKVD+ LF+LILAANYL+IK LLDLTCQ AD++KGK EE+R FNIKND
Sbjct: 315 LKNWDADFVKVDKEPLFELILAANYLDIKSLLDLTCQAAADVMKGKKVEEVRTMFNIKND 494
Query: 393 FTP 385
+TP
Sbjct: 495 YTP 503
Score = 88.6 bits (187), Expect(2) = 4e-048
Identities = 37/57 (64%), Positives = 41/57 (71%)
Frame = -2 / +3
Query: 849 KKMITLKSSDGXXXXXXXXXXXESQTIRHMIEDDCADNGIPLPNVNSKILSKVIEYC 679
KKMITLKS DG ESQTI+HMIEDDCADN IP+ NV K+L+KVIEYC
Sbjct: 87 KKMITLKSFDGETFEVSEAVALESQTIKHMIEDDCADNAIPIQNVTGKVLAKVIEYC 257
Score = 79.0 bits (166), Expect(2) = 4e-026
Identities = 40/63 (63%), Positives = 46/63 (73%)
Frame = +2 / -2
Query: 386 GVKSFLMLKVLRISSGVLPLIMSATV*QVRSSRPLMFR*FAAKMRSKSVAWSTLTKSASQ 565
GV SFLML ++R SS P I SA QVRSSR L+ +*FAA+M SKS + ST TKSASQ
Sbjct: 502 GV*SFLMLNIVRTSSTFFPFITSAAAWQVRSSRLLISK*FAARMSSKSGSLSTFTKSASQ 323
Query: 566 FLR 574
FLR
Sbjct: 322 FLR 314
Score = 53.8 bits (111), Expect(2) = 4e-026
Identities = 27/57 (47%), Positives = 31/57 (54%)
Frame = +2 / -2
Query: 677 LQYSITLDRILELTLGRGMPLSAQSSSIMWRMVCDXXXXXXXXXXXXXXELLRVIIF 847
LQYSIT L +T G+ LSAQSSSIM +VCD +L RVIIF
Sbjct: 259 LQYSITFANTLPVTF*IGIALSAQSSSIMCLIVCDSSATASETSKVSPSKLFRVIIF 89
>gb|CO513276.1|CO513276 s13dSG37B0100009_129716 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 99.6 bits (211), Expect(2) = 1e-043
Identities = 40/46 (86%), Positives = 45/46 (97%)
Frame = -2 / +1
Query: 579 EDLKNWDADFVKVDQATLFDLILAANYLNIKGLLDLTCQTVADMIK 442
++LK+WD++FVKVDQATLFDLILAANYLNIK LLDLTCQTVADMIK
Sbjct: 358 DELKSWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCQTVADMIK 495
Score = 91.8 bits (194), Expect(2) = 1e-043
Identities = 39/56 (69%), Positives = 43/56 (76%)
Frame = -2 / +1
Query: 846 KMITLKSSDGXXXXXXXXXXXESQTIRHMIEDDCADNGIPLPNVNSKILSKVIEYC 679
+ ITLKSSDG ESQTI+HMIEDDCAD+GIPLPNV SKIL+KVIEYC
Sbjct: 145 RKITLKSSDGETFEVDEAVALESQTIKHMIEDDCADSGIPLPNVTSKILAKVIEYC 312
Score = 68.0 bits (142), Expect(2) = 5e-025
Identities = 24/41 (58%), Positives = 35/41 (85%)
Frame = +1 / -1
Query: 442 LDHVSNSLTGQVEQTLDVQIVCSQDEVEKRGLVDLDEVRIP 564
LDHV NSL G+++Q LD+Q++ SQDE++KR L++LDE+ IP
Sbjct: 495 LDHVCNSLAGKIQQALDIQVISSQDEIKKRSLINLDELGIP 373
Score = 61.1 bits (127), Expect(2) = 5e-025
Identities = 30/55 (54%), Positives = 35/55 (63%)
Frame = +2 / -2
Query: 677 LQYSITLDRILELTLGRGMPLSAQSSSIMWRMVCDXXXXXXXXXXXXXXELLRVI 841
LQYSIT +IL +TLGRG+PLSAQSSSI+ +V D ELLRVI
Sbjct: 314 LQYSITFAKILLVTLGRGIPLSAQSSSIICLIV*DSKATASSTSKVSPSELLRVI 150
>gb|CO517166.1|CO517166 s13dSG33D0700062_474416 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 553
Score = 149 bits (319), Expect = 7e-037
Identities = 63/78 (80%), Positives = 68/78 (87%)
Frame = -2 / -3
Query: 570 KNWDADFVKVDQATLFDLILAANYLNIKGLLDLTCQTVADMIKGKTPEEIRKTFNIKNDF 391
K+WD++FVKVDQATLFDLILAANYLNIK LLDLTC+TVA MIKGKTPEEIRKTFNIKNDF
Sbjct: 551 KSWDSEFVKVDQATLFDLILAANYLNIKSLLDLTCRTVAAMIKGKTPEEIRKTFNIKNDF 372
Query: 390 TPXXXXXXXXENQWAFE* 337
+P ENQWAFE*
Sbjct: 371 SPEEEEEVRRENQWAFE* 318
Score = 126 bits (270), Expect = 4e-030
Identities = 61/78 (78%), Positives = 66/78 (84%)
Frame = +2 / +1
Query: 338 YSKAHWFSLRISSSSSGVKSFLMLKVLRISSGVLPLIMSATV*QVRSSRPLMFR*FAAKM 517
+S AHWFSLR SSSSSG KSFLML VLRISSGV PLIM+ATV QVRSS+ L+F+* AAKM
Sbjct: 319 HSNAHWFSLRTSSSSSGEKSFLMLNVLRISSGVFPLIMAATVRQVRSSKLLIFK*LAAKM 498
Query: 518 RSKSVAWSTLTKSASQFL 571
RSKSVA STLT S SQ L
Sbjct: 499 RSKSVA*STLTNSESQDL 552
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,391,125
Number of Sequences: 7669
Number of extensions: 74293
Number of successful extensions: 906
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 251751474
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)