TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2542042.2.1
(691 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU978120.1|BU978120 HA12J22u HA Hordeum vulgare subsp. v... 71 5e-026
gb|BQ657192.1|BQ657192 HA09F08u HA Hordeum vulgare subsp. v... 71 5e-026
gb|BU966694.1|BU966694 HB02B05r BC Hordeum vulgare subsp. v... 72 2e-024
>gb|BU978120.1|BU978120 HA12J22u HA Hordeum vulgare subsp. vulgare cDNA clone HA12J22
3-PRIME, mRNA sequence
Length = 447
Score = 70.7 bits (148), Expect(2) = 5e-026
Identities = 23/40 (57%), Positives = 32/40 (80%)
Frame = +2 / -1
Query: 326 TAIAMCHANTMNWDDRYFQMLNVTRGEEICHFMPRNYVLW 445
TAIAMCH NT +WD +YF++L+ GE ICH+MP NY+++
Sbjct: 198 TAIAMCHMNTSDWDTQYFELLDGKHGEPICHYMPTNYIMF 79
Score = 66.6 bits (139), Expect(2) = 5e-026
Identities = 22/35 (62%), Positives = 29/35 (82%)
Frame = +2 / -1
Query: 212 VVPCHPMSYPYMVHYCHQPADVEALRIELTGLGDG 316
VVPCHPM +PY V YCH+P +V++LR++L GL DG
Sbjct: 315 VVPCHPMDFPYEVFYCHRPKEVQSLRVQLKGLKDG 211
>gb|BQ657192.1|BQ657192 HA09F08u HA Hordeum vulgare subsp. vulgare cDNA clone HA09F08
3-PRIME, mRNA sequence
Length = 404
Score = 70.7 bits (148), Expect(2) = 5e-026
Identities = 23/40 (57%), Positives = 32/40 (80%)
Frame = +2 / -2
Query: 326 TAIAMCHANTMNWDDRYFQMLNVTRGEEICHFMPRNYVLW 445
TAIAMCH NT +WD +YF++L+ GE ICH+MP NY+++
Sbjct: 205 TAIAMCHMNTSDWDTQYFELLDGKHGEPICHYMPTNYIMF 86
Score = 66.6 bits (139), Expect(2) = 5e-026
Identities = 22/35 (62%), Positives = 29/35 (82%)
Frame = +2 / -2
Query: 212 VVPCHPMSYPYMVHYCHQPADVEALRIELTGLGDG 316
VVPCHPM +PY V YCH+P +V++LR++L GL DG
Sbjct: 322 VVPCHPMDFPYEVFYCHRPKEVQSLRVQLKGLKDG 218
>gb|BU966694.1|BU966694 HB02B05r BC Hordeum vulgare subsp. vulgare cDNA clone HB02B05
5-PRIME, mRNA sequence
Length = 438
Score = 72.1 bits (151), Expect(2) = 2e-024
Identities = 23/40 (57%), Positives = 32/40 (80%)
Frame = +2 / +1
Query: 326 TAIAMCHANTMNWDDRYFQMLNVTRGEEICHFMPRNYVLW 445
TA AMCH NT +WD++YF++L RGE ICH+MP NY+++
Sbjct: 235 TATAMCHMNTSDWDEQYFELLGGVRGEPICHYMPTNYIMF 354
Score = 59.7 bits (124), Expect(2) = 2e-024
Identities = 20/35 (57%), Positives = 27/35 (77%)
Frame = +2 / +1
Query: 212 VVPCHPMSYPYMVHYCHQPADVEALRIELTGLGDG 316
VVPCHPM +PY V YCH+P +V++L ++L L DG
Sbjct: 118 VVPCHPMDFPYEVFYCHRPKEVQSLTVQLKVLEDG 222
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 278,820,774
Number of Sequences: 312970
Number of extensions: 3807190
Number of successful extensions: 44429
Number of sequences better than 1.0e-020: 3
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7550174202
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)